2015 Fiscal Year Final Research Report
Investigation of novel intracellular function of interaction between CCN2 and Rab14 in bone and cartilage cells
| Project/Area Number |
26893161
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Okayama University |
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Principal Investigator |
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| Project Period (FY) |
2014-08-29 – 2016-03-31
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| Keywords | 骨・軟骨組織 / 細胞内輸送 / Rab14 / CCN2 |
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| Outline of Final Research Achievements |
The aim of this study is to identify interactive domain between CCN2 and Rab14, and to investigate its role in the physiological activity in bone and cartilage cells. The studies revealed the following: .....
1) CCN2 bound to Rab14 through its IGFBP domain among the four domains in CCN2. 2) CCN2 and Rab14 co-localized in cells, and regulated in intracellular membrane trafficking. 3) ER and Golgi stresses were increased by suppressing the expression of rab14 and ccn2 mRNA. 4) A dominant negative form of Rab14 overexpression decreased proteoglycan accumulation in HCS-2/8 cells.
These results suggest that intracellular CCN2 is associated with Rab14 on proteoglycan-containing vesicles during their transport from golgi to endosomes in chondrocytes and that the association play some role in proteoglycan synthesis by chondrocyte.
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| Free Research Field |
歯科矯正学
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