Evaluation of angiogenesis and bone regeneration in osteonecrosis using bone marrow mononuclear cells transfected by microRNAs.

2015 Fiscal Year Final Research Report

• Project/Area Number: 26893163
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Hiroshima University
• Principal Investigator: SHOJI TAKESHI 広島大学, 病院(医), 病院助教 (50736569)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Keywords: 大腿骨頭壊死症 / microRNA / エクソソーム / 骨髄単核球 / 血管再生

Outline of Final Research Achievements

The comprehensive analysis of miRNA and mRNA expression were performed using the femoral head that was obtained from the patient with osteonecrosis of femoral head. I evaluated the ability of osteogenic proliferation and vascularization using miRNA candidates, and lined up four candidates that ware considered to be useful for angiogenesis and bone regeneration. I also revealed that microRNA can be transfected into cells in other cultured cells such as myoblasts with exosomes that contained synthetic miRNA that was obtained from microRNA transfected mesenchymal stem cells, and microRNA increased density-dependently.

Free Research Field

関節病学