Periodontal regenerative effects by inhibition of Sprouty2 protein in periodontal ligament cells

2015 Fiscal Year Final Research Report

• Project/Area Number: 26893196
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Periodontology
• Research Institution: Kyushu University
• Principal Investigator: Tanaka Urara 九州大学, 歯学研究科(研究院), 研究員 (50734993)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Keywords: Spry2 / proliferation / migration / bFGF / EGF / periodontal regeneration

Outline of Final Research Achievements

In this study, we investigated how Spry2 inhibition affects the cellular physiology of periodontal ligament (PDL) cells. ERK1/2 activation and proliferation of 1-17 PDL cells were significantly upregulated by the addition of Spry2 siRNA in the presence of bFGF and EGF. In addition, Spry2 siRNA reduced transcription of osteogenesis-related genes and ALP staining relative to control cells. Furthermore, it increased AKT/phosphatidylinositol 3-kinase (PI3K) phosphorylation; consequently, Rac1 but not Cdc42 was activated, thereby promoting lamellipodia formation, cell proliferation, and migration after stimulation by bFGF and EGF. In conclusion, Spry2 combined with bFGF and EGF stimulation reduced PDL cell migration and proliferation with inducing osteoblastic differentiation. These in vitro findings may provide a molecular basis for novel therapeutic approaches for establishing periodontal tissue regeneration.

Free Research Field

periodontology