Vasculogenic conditioned peripheral blood mononuclear cells rescue the radiation-inducedsalivary glandhypofunction

2015 Fiscal Year Final Research Report

• Project/Area Number: 26893199
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: Nagasaki University
• Principal Investigator: I Takashi 長崎大学, 医歯薬学総合研究科(歯学系), 助教 (30733448)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Keywords: 唾液腺

Outline of Final Research Achievements

We have tried to develop the cell therapy with the peripheral blood-derived mononuclear cells (PBMNCs), purified by recently improved quality and quantity (QQ) culture system of EPCs. In vivo, QQ-PBMNCs were transplanted to SG of the mice directly after irradiation. In vitro, we reveled that QQ-PBMNCs contained angiovasculogenesis and anti-inflammatory cell population abundantly. In vivo, SGs of treated mice were observed decreased area of damaged acinar cells and fibrosis. Donor cells were detected mainly around the blood vessels in SGs, and QQ-PBMNCs-treated SGs showed more expression of proliferating cell nuclear antigen and stem cell markers. Otherwise, inflammatory gene expression were downregulated. Remarkably, SGs of treated mice showed the increased level of blood vessel formation gradually. Our data suggests that cell therapy with QQ-PBMNCs can prevent the radiation-induced SG hypofunction by their abilities of vascular regeneration and anti-inflammation.

Free Research Field

再生医学