Somatic cell fate conversion by reprogramming factor isolated from Lactic acid bacteria

2015 Fiscal Year Final Research Report

• Project/Area Number: 26893200
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Kumamoto University
• Principal Investigator: Ito Naofumi 熊本大学, 大学院生命科学研究部(医), 研究員 (60732716)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Keywords: 発生 / 細胞分化 / 初期化 / リボソーム / 多能性幹細胞

Outline of Final Research Achievements

In previous studies, by adding lactic acid bacterium cells to human dermal fibroblasts induced characteristic cell mass formation was revealed. The cell mass had a multiple differential ability beyond germ layers limit. Chromatographic fractionated lactic acid bacteria lysate was added to the human skin cells result the cell mass formation and identified high activity condition for the cell mass formation. The fraction was analyzed by mass spectrometer and identified few proteins. It was also found that the molecule of interest is a molecule of above 100 kDa by other biochemical analysis. It was identified that the molecule of interest is a ribosome.

Free Research Field

発生生物学