Transcriptional regulation of XI/XXVII collagen gene in chondrocyte

2015 Fiscal Year Final Research Report

• Project/Area Number: 26893208
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Oita University
• Principal Investigator: Hida Mariko 大分大学, 医学部, 客員研究員 (10737224)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Keywords: 軟骨 / コラーゲン / 転写

Outline of Final Research Achievements

We investigated the proximal promoter of mouse Col11a1 gene in chondrocytes. Cell transfection experiments with reporter constructs in the absence of NF-Y binding sequence exhibited the suppression of the promoter activity. Furthermore, NF-Y protein directly bound to this region in vivo, and dominant-negative NF-Y A mutant inhibited the promoter activity. Next, we further characterized the proximal promoter activity of mouse Col11a1 gene. Luciferase assays exhibited that GC rich sequence is a critical elements of Col11a1 promoter activity, and the most effective candidate was transcription factor Sp1. Overexpression of Sp1 was significantly increased the activity and knockdown of Sp1 expression was suppressed the proximal promoter activity and the expression of endogenous transcript of mouse Col11a1 gene. Taken together, these results indicate that the transcription factor NF-Y and Sp1 upregulates the proximal promoter activity of mouse Col11a1 gene in chondrocytes.

Free Research Field

分子生物学