2015 Fiscal Year Final Research Report
Development of artificial epididymosome (ARTEPS) and elucidation of the sperm capacitation mechanism
Project/Area Number |
26893216
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Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Single-year Grants |
Research Field |
General medical chemistry
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Research Institution | Ibaraki Prefectural University of Health Science |
Principal Investigator |
KATOH YUKI 茨城県立医療大学, 公私立大学の部局等, 研究員 (60733649)
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Project Period (FY) |
2014-08-29 – 2016-03-31
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Keywords | 精子 / 受精能獲得 / エピディディモソーム / 精子成熟 |
Outline of Final Research Achievements |
In mammals, only the sperm that have undergone stepwise activation, including maturation in the epididymis and capacitation (CPN) in the tubal isthmus, are able to initiate both hyperactivated motility and acrosome reaction to fertilize an ovum. Although the mechanisms in CPN remain unclear, the tyrosine phosphorylation of functional proteins and the production of reactive oxygen species (ROS), are thought to be particularly important. In the present study, I first performed the functional analyses of aldose reductase (AR) and NADP+ isocitrate dehydrogenase (IDPc) that were tyrosine-phosphorylated during the capacitation. Second, I tried to develop the protein delivery system to immature sperm using artificially formed epididymosome.
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Free Research Field |
生殖生化学
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