Pathophysiological significance of Angiotensin Receptor-binding Molecule in progression of renal dysfunction

2015 Fiscal Year Final Research Report

• Project/Area Number: 26893218
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Kidney internal medicine
• Research Institution: Yokohama City University
• Principal Investigator: OHSAWA masato 横浜市立大学, 医学(系)研究科(研究院), 客員研究員 (60733433)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Keywords: 腎障害 / レニン・アンジオテンシン系 / 尿細管 / 腎臓 / 高血圧

Outline of Final Research Achievements

The renin-angiotensin system plays a key role in the maintenance of cardiovascular and renal homeostasis. We previously identified an AT1R-associated protein (ATRAP), which is a molecule directly interacting with the AT1R. The present study was performed to investigate pathophysiological significance of ATRAP in mice model of CKD by employing systemic ATRAP-KO mice. ATRAP-KO mice and their WT mice were subjected to unilateral ureteral obstruction (UUO) or 5/6 nephrectomy (Nx) as CKD model. To examine the effect of ATRAP deficiency, parameters of renal function and fibrosis, proinflammatory cytokine, blood pressure were measured in ATRAP-KO mice and WT mice after UUO or 5/6 Nx. Expression of endogenous ATRAP and proinflammatory cytokine were changed in WT mice after UUO or 5/6 Nx. Accumulating results indicate that ATRAP possibly exerts inhibitory effects on exacerbated AT1R activation in response to pathological stimuli in CKD model.

Free Research Field

腎臓内科学