The development of minimally invasive bone regeneration with recombinant growth factor enhanced the bone regeneration effect

2015 Fiscal Year Final Research Report

• Project/Area Number: 26893248
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Prosthodontics/ Dental materials science and
• Research Institution: Iwate Medical University
• Principal Investigator: Jun Yokota 岩手医科大学, 歯学部, 助教 (60733730)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Keywords: 遺伝子導入 / 骨再生

Outline of Final Research Achievements

In this study, to affect growth factors to the mesenchymal stem cells (MSC), we transfected plasmid vector, and evaluate the osteogenic differentiation. And we also investigated the effects of TGF-β and VEGF on the osteogenic differentiation of MSC. As a result, BMP-2 plasmid vector induced matrix mineralization in the MSC in a dose-dependent manner.
TGF-β and VEGF stimulated to the MSC, cell proliferation was downregulated, but osteo differentiation markers (ALP, BSP, OCN) expression was significantly upregulated. Furthermore TGF-β and VEGF induced extracellular signal-regulated kinase (ERK) phosphorylation. These findings indicated that gene transfection and the combination of TGF-β and VEGF were significantly differentiated MSC into osteoblasts, and our findings provide insight into the establishment of novel therapeutic methods for bone formation.

Free Research Field

再生医療