Comprehensive analysis of microRNAs using patients' tissues with acute myocardial infarction.

2015 Fiscal Year Final Research Report

• Project/Area Number: 26893278
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Legal medicine
• Research Institution: Tokai University
• Principal Investigator: KAKIMOTO Yu 東海大学, 医学部, 助教 (40734166)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Keywords: miRNA / 心房 / 心室 / ヒト組織 / 解剖検体 / 心筋梗塞

Outline of Final Research Achievements

(1)MiRNAs were stably detected in the autoptic cases, where the bodies were moved to the morgue within a day and the tissues were sampled within a week. (2)Under formalin fixation, miRNAs were more stably detected than longer RNAs, but decreased depending on the fixation period. (3)MiRNAs in FFPE tissues within 2-month fixation could be quantified by real-time PCR with the miRNA-size control. (4)Among 1,134 miRNAs identified by next-generation sequencing, 14 miRNAs were upregulated in atrium, and 11 miRNAs were upregulated in ventricle. (5)MiR-208 family showed significant chamber-specificity in the human heart.

Free Research Field

法医学