1985 Fiscal Year Final Research Report Summary
Production of human renin in microorganism and its application to medicine.
| Project/Area Number |
58440078
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
医学一般(含病院管理学・看護学・人類遺伝学・病態検査学・実験動物)
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
1983 – 1985
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| Keywords | Renin / Prorenin / cDNA / 大腸菌 / 発現 |
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| Research Abstract |
1) The primary structure of human renin precursor was deduced from its complementary sequence. The predicted sequence consists of 406 amino acids with a pre- and a prosegment carrying 20 and 46 amino acids, respectively.
2) A three-dimensional model of human renin has been constructed based on the assumption that the overall folding of the aspartyl proteases is very similar. As a reference, we used penicillopepsin.
3) The human renin gene was isolated from a Charon 4A human genomic library and characterized. The gene spans about 11.7 kilobases and consists of 10 exons and 9 introns that map at points that could be variable surface loops of the enzyme.
4) Human prorenin was produded under the transcriptional control of the E. coli trp promoter-operator in the expression vector. The transformed E. coli cell expressed high levels (30% of total cell protein) of prorenin as part of a hybrid protein.
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