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1985 Fiscal Year Final Research Report Summary

Fermentative Production of Pyrroloquinoline Quinone

Research Project

Project/Area Number 58860015
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field 発酵・醸造
Research InstitutionYamaguchi University

Principal Investigator

AMEYAMA Minoru  山口大学, 農, 教授 (90022053)

Project Period (FY) 1983 – 1985
KeywordsPyrroloquinoline quinone / Quinoproteins / 補酵素の発酵生産 / PQQの精製
Research Abstract

The possibility of preparing pyrroloquinoline quinone (PQQ), a novel coenzyme, by a fermentative method was proposed and established a simple method for PQQ fermentation. PQQ was formed and accumulated in the culture medium of various microorganisms. Some methylotrophs were most favorable for PQQ production and about 100 ug/ml of PQQ was found in the culture medium after 2-3 days incubation. Methanol was utilized effectively for PQQ formation and 1-3% of methanol in the culture medium was found to be optimal. It was also found that the regulation in PQQ formation was accompanied with magnesium concentration of the medium.
An attempt at the isolation and purification of PQQ was made successfully and spectral evidence was obtained suggesting that the isolated compound was identical with authentic PQQ.
By the way, an improved enzymatic method for the determination of PQQ was developed with cytoplasmic membrane of Escherichia coli, in which D-glucose dehydrogenase (EC 1.1.99.17), a quinoprotein, was completely resolved to apoenzyme by EDTA treatment. Incubation of the EDTA-treated membrane with exogenous PQQ by assaying the restored D-glucose dehydrogenase activity.

  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] 日本農芸化学会欧文誌. 48-2. (1984)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 日本農芸化学会欧文誌. 48-11. (1984)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 日本農芸化学会欧文誌. 48-12. (1984)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 日本農芸化学会欧文誌. 49-3. (1985)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 日本農芸化学会欧文誌. 50-1. (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Agric. Biol. Chem.48(2). 561-565 (1984)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Agric. Biol. Chem.48(11). 2873-2876 2909-2911 (1984)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Agric. Biol. Chem.48(12). 3099-3107 (1984)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Agric. Biol. Chem.49(3). 699-709 853-854 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Agric. Biol. Chem.49(4). 1227-1231 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Agric. Biol. Chem.49(12). 3623-3626 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Anal. Biochem.151(2). 263-267 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Agric. Biol. Chem.50(1). 49-57 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J. Brewing Soc. Jpn.79(10). 717-720 (1984)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chemistry Today. No. 172. 46-52 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jpn. Soc. Vitaminol.59(12). 640-642 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] The Enzymes, a guidline to biotechnology. Asakura-shoten, (1985)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1988-11-09  

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