1985 Fiscal Year Final Research Report Summary
Ultramicrotracing of Biological Active Substances by High Performance Liquid Chromatography
| Project/Area Number |
58870103
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical pharmacy
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Project Period (FY) |
1983 – 1985
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| Keywords | High performance liquid chromatography / fluorometric analysis / guanidinecontaining peptide / thiol-containing peptide / catecholamine / steroid / bile acid sulfate / 1-ブロモアセチルピレン / 1-アンスロイルニトリル |
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| Research Abstract |
Achievements of the project is as follows:
Kinoshita established a method for determination of bradykinine using alkaline ninhydrin reagent and applied it to the studies on enzymatic degradation of gradykinine in human plasma. Nimura established a method for determination of glutathione in human blood using o-phthalaldehyde reagent. Diluted blood could be directly injected into a chromatograph without pre-treatment. Imai developed a method for determination of catecholamines catecholamines and L-dopa using ethylenediamine and applied it to monitor these substances in biological samples. Tsuji synthesized 1-bromoacetylpyrene for the fluorescent labeling of carboxyl group and employed it to estimate sulfateconjugated bile acids in biological fluids. Nambara prepared 9- or 1-anthroylnitrile as a fluorescent labeling reagents for hydroxy group and applied it to the determination of various steroids and bile acids. Ohkura devised a method to determine histidine decarboxilase activity by measuring histamine fluorometrically.
New determination methods established in this research project were extremely effective for the ultramicrotracing of such biological materials.
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