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1985 Fiscal Year Final Research Report Summary

Ultramicrotracing of Biological Active Substances by High Performance Liquid Chromatography

Research Project

Project/Area Number 58870103
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field Physical pharmacy
Research InstitutionKitasato University

Principal Investigator

KINOSHITA Toshio  北里大学, 薬, 教授 (70053816)

Project Period (FY) 1983 – 1985
KeywordsHigh performance liquid chromatography / fluorometric analysis / guanidinecontaining peptide / thiol-containing peptide / catecholamine / steroid / bile acid sulfate / 1-ブロモアセチルピレン / 1-アンスロイルニトリル
Research Abstract

Achievements of the project is as follows:
Kinoshita established a method for determination of bradykinine using alkaline ninhydrin reagent and applied it to the studies on enzymatic degradation of gradykinine in human plasma. Nimura established a method for determination of glutathione in human blood using o-phthalaldehyde reagent. Diluted blood could be directly injected into a chromatograph without pre-treatment. Imai developed a method for determination of catecholamines catecholamines and L-dopa using ethylenediamine and applied it to monitor these substances in biological samples. Tsuji synthesized 1-bromoacetylpyrene for the fluorescent labeling of carboxyl group and employed it to estimate sulfateconjugated bile acids in biological fluids. Nambara prepared 9- or 1-anthroylnitrile as a fluorescent labeling reagents for hydroxy group and applied it to the determination of various steroids and bile acids. Ohkura devised a method to determine histidine decarboxilase activity by measuring histamine fluorometrically.
New determination methods established in this research project were extremely effective for the ultramicrotracing of such biological materials.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Experientia. 41. (1985)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Anal.Biochem.146. (1985)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] J.Chromatogr.328. (1985)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] J.Chromatogr.345. (1985)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] J.Chromatogr.311. (1984)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Experimentia. 41. (1985)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Anal. Biochem.146. (1985)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J. Chromatogr.328. (1985)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J. Chromatogr.345. (1985)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J. Chromatogr.311. (1984)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1988-11-09  

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