1986 Fiscal Year Final Research Report Summary
Phylogeny of 5S RNA and evolution of ribosomal components
| Project/Area Number |
59400007
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
広領域
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| Research Institution | Nagoya University |
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Principal Investigator |
OSAWA Syozo Nagoya University, 理学部, 教授 (10034620)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAO Fumiaki Nagoya University, 理学部, 助手 (10158074)
HORI Hiroshi Nagoya University, 理学部, 助手 (60116663)
MUTO Akira Nagoya University, 理学部, 助教授 (80034635)
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| Project Period (FY) |
1984 – 1986
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| Keywords | Mycoplasma / Micrococcus / GC / AT pressure / Genetic code / Mutation pressure / UGA tryptophan codon / tRNA / Micrococcus |
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| Research Abstract |
1.Since 1984, we have been studying the phylogenic relationship among a wide spectrum of extant living organisms using 5S RNA sequences. By comparing more than 350 5S RNA sequences from widely separated organisms, we have constructed a phylogenetic tree to obtain information on the representative groups of organisms, such as eubacteria, plants, multicellular animals, etc.
2. We proposed a hypothesis for evolution of the genetic code, using conclusions based on experimental observations. We emphasize wobble rules, evolution of anticodons and of transfer RNA, especially through gene duplication, AT/GC pressure in DNA (mutation pressure), observed differences from the universal code in mitochondria, Mycoplasma (UGA : tryptophan codon), and ciliated protozoa, and phylogenetic separation of kingdoms of organisms deduced from the 5S RNA sequences.
Consideration of these and other observations including codon usages in ribosomal proteins in Micrococcus and Mycoplasma done in our laboratory enables a coherent scheme for evolution of the code, following the first establishment of a code for 20 amino acids, to be formulated.
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Research Products
(11 results)
