1985 Fiscal Year Final Research Report Summary
On formation and chemical structure of functional oligasaccharide from lactose
| Project/Area Number |
59430022
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
畜産化学
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
1984 – 1985
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| Keywords | galacto-oligosaccharire / <beta> -galactosidase / ラクトース |
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| Research Abstract |
Di-, tri-, tetra-, penta- and hexasaccharides were formed during the hydrolysis of lactose by transgalactosylation reaction of Aspergillus oryzae <beta> -galactosidase. In this study the isolation and characterization of the major constituents of tri-, tetra- and pentasaccharides are described. The structure elucidation of 3 tri-, 2 tetra- and 1 pentasaccharides was carried out by methylation analysis, mass spectrometry and <^(13)C> -nmrspectrometry. The trisaccharides are 3'-galactosyl-lactose, 6'-galactosyl -lactose and 4,6-digalactosyl-glucose. Tetrasaccharides are O- <beta> -D-galacto pyranosyl-(1-6)-O- <beta> -D-galactopyranosyl-(1-6)-O- <beta> -D-galactopyranosyl-(1-4)-D-glucose and O- <beta> -D-galactopyranosyl-(1-6)-O- <beta> -D-galactopyranosyl-(1-3) [or O- <beta> -D-galactopyranosyl-(1-3)-O- <beta> -D-galactopyranosyl-(1-6)]-O- <beta> -D-galactopyranosyl-(1-4)-D-glucose. Pentasaccharide is O- <beta> -D-galactopyranosyl-(1-6)-O- <beta> -D-galactopyranosyl-(1-6)-O- <beta> -D-galactopyranosyl-(1-4)-D-glucose.
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