1986 Fiscal Year Final Research Report Summary
A Cytobiochemical Characterization of Cell Diferentiation Derived from Calcified and Non-calcified Tissues.
| Project/Area Number |
59440078
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional basic dentistry
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| Research Institution | Kanagawa Dental College |
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Principal Investigator |
SAITO Shigeru Kanagawa Dental College, Professor, 歯学部, 教授 (80084713)
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| Co-Investigator(Kenkyū-buntansha) |
NAKANO Tamotsu Kanagawa Dental College, Associate Researcher, 歯学部, 助手 (80164250)
IMAI Kiyoshi Kanagawa Dental College, Associate Researcher, 歯学部, 助手 (10151656)
NISHIYAMA Katsuhiro Kanagawa Dental College, Associate Researcher, 歯学部, 助手 (20084783)
KAWASE Toshio Kanagawa Dental College, Assistant Professor, 歯学部, 助教授 (30084784)
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| Project Period (FY) |
1984 – 1986
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| Keywords | Calcification / Alkaline phosphatase / 1,25 <(OH)_2> <D_3> / Dexamethasone / Monoclonal antibody for human periodontal ligament / Rat calvaria osteoblast / 歯周組織由来細胞 / モノクローナル抗体 |
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| Research Abstract |
The mechanism of calcification was investigated in terms of characteristic cell function of periodontal ligament cell (PLF) and fibroblast derived from non-calcified tissues in comparison with osteogenic cells from rat pup calvaria and bone in which extensive researches had been accumulated during past decays.
The periodontal ligament is mainly of fibrous tissue containing various non-collagenous components, which tightly connects the tooth to the alveolar bone. Although the oxygen uptake of periodontal ligament was measured as 1/50 to that liver ( <Q_O2> /DNA=0.2), the metabolic activities of amino acids, proteins and glycosaminoglycans synthesis were found extensive through transamination and gluconeogenesis of intermediates converted from succinate-1,4- <^(14)C> which was added in the medium of short-term incubation system in vitro. Bovine PLF has a remarkably high activity of alkaline phosphatase (ALPase) compared to skin fibroblasts, which seem to have similar, but not identical phenotype with that of the rat osteoblast-like cells. The ALPase prepared from the human PLF and alveolar bone exhibited the same enzymatic character with bovine PLF. It is now possible to refer to PLF as an osteoblastic fibroblast and has proposed this as a work hypothesis. Important findings of this research are that ALPase activity of PLF and bone cells was regulated by 1,25 <(OH)_2> <D_3> and dexamethasone, and also 66Kd phosphoprotein was found as dose responding protein of active <D_3> within physiological concentration, which may have an important role for the transformation of octacalcium phosphate. Furtheremore, three stains of monoclonal antibodies synthesized cell line for human PLF were obtained by the cell fusion using myenoma NS-S.
Those results mentioned above provide new aspect for the processes of calcification which mainly consists of inhibitory mechanism of mineralization observed in periodontal ligament.
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