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1986 Fiscal Year Final Research Report Summary

A Cytobiochemical Characterization of Cell Diferentiation Derived from Calcified and Non-calcified Tissues.

Research Project

Project/Area Number 59440078
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionKanagawa Dental College

Principal Investigator

SAITO Shigeru  Kanagawa Dental College, Professor, 歯学部, 教授 (80084713)

Co-Investigator(Kenkyū-buntansha) NAKANO Tamotsu  Kanagawa Dental College, Associate Researcher, 歯学部, 助手 (80164250)
IMAI Kiyoshi  Kanagawa Dental College, Associate Researcher, 歯学部, 助手 (10151656)
NISHIYAMA Katsuhiro  Kanagawa Dental College, Associate Researcher, 歯学部, 助手 (20084783)
KAWASE Toshio  Kanagawa Dental College, Assistant Professor, 歯学部, 助教授 (30084784)
Project Period (FY) 1984 – 1986
KeywordsCalcification / Alkaline phosphatase / 1,25 <(OH)_2> <D_3> / Dexamethasone / Monoclonal antibody for human periodontal ligament / Rat calvaria osteoblast / 歯周組織由来細胞 / モノクローナル抗体
Research Abstract

The mechanism of calcification was investigated in terms of characteristic cell function of periodontal ligament cell (PLF) and fibroblast derived from non-calcified tissues in comparison with osteogenic cells from rat pup calvaria and bone in which extensive researches had been accumulated during past decays.
The periodontal ligament is mainly of fibrous tissue containing various non-collagenous components, which tightly connects the tooth to the alveolar bone. Although the oxygen uptake of periodontal ligament was measured as 1/50 to that liver ( <Q_O2> /DNA=0.2), the metabolic activities of amino acids, proteins and glycosaminoglycans synthesis were found extensive through transamination and gluconeogenesis of intermediates converted from succinate-1,4- <^(14)C> which was added in the medium of short-term incubation system in vitro. Bovine PLF has a remarkably high activity of alkaline phosphatase (ALPase) compared to skin fibroblasts, which seem to have similar, but not identical phenotype with that of the rat osteoblast-like cells. The ALPase prepared from the human PLF and alveolar bone exhibited the same enzymatic character with bovine PLF. It is now possible to refer to PLF as an osteoblastic fibroblast and has proposed this as a work hypothesis. Important findings of this research are that ALPase activity of PLF and bone cells was regulated by 1,25 <(OH)_2> <D_3> and dexamethasone, and also 66Kd phosphoprotein was found as dose responding protein of active <D_3> within physiological concentration, which may have an important role for the transformation of octacalcium phosphate. Furtheremore, three stains of monoclonal antibodies synthesized cell line for human PLF were obtained by the cell fusion using myenoma NS-S.
Those results mentioned above provide new aspect for the processes of calcification which mainly consists of inhibitory mechanism of mineralization observed in periodontal ligament.

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] T.Kawase;S.Sato;A.Miake;S.Saito: J.Dent.Res.(1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.W.Neuman;K.Imai;T.Kawase;S.Saito: Bone and Mineral. 2. (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Sugizaki;K.Imai;M.Kimura;K.Tanikawa;H.Inoue;T.Kawase;K.Okuda;I.Takazoe;S.Saito: Bull.of Kanagawa Dental col.15. (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 木村守隆,谷川好一,佐竹司,中野完,川瀬俊夫,斎藤滋: 神奈川歯学. (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 浜田三郎,木本茂成,浜田作光,葉山淑人,丹下貴司,檜垣旺夫,川瀬俊夫,斎藤滋: 日本小児歯科学会誌. 25(2). (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 今井喜良,弓削公,松本徹,川瀬俊夫,伊藤節郎,田中千禾夫,斎藤滋: 生体材料. (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T. Kawase et al: "Alkaline Phosphatase of Human Periodontal Ligament Fibroblast-like cells in vitro" J. Dent. Res.(1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.W.Neuman et al: "The Calcium Buffering Phase Neonatl Rat Bone Mineral Some Clues to its Form and Formation." Bone and Minaral. 2. (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Sugizaki et al: "Extraction of Lipopolysaccharides from Preiodontopathic Bacteria." Bull. of Kanagawa Dental Col.15. (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Kimura et al: "Mechanisms of Cell Adhesion on Bioglass-Ceramic Composites" J. Kanagawa Odontological Society. (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Hamada et al: "Cell Culture of Bovine Tooth Germ: The Effect of Vitamin <D_3> on Cells of Dental Sac." J. Japanese Pedodontic Association. 25(2). (1987)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1988-11-09  

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