1985 Fiscal Year Final Research Report Summary
DNA Repair of Ionizing Radiation Damage in Human and Experimental Animal.
| Project/Area Number |
59440093
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
放射線5生物学
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
1984 – 1985
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| Keywords | Ionizing radiation / Ataxia telangiectasia / 電離放射線 / 胎盤抽 / 出物 / 疫学 |
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| Research Abstract |
1. Objective:
Ionizing radiation gives rather non-specific damage to DNA and the repair of damage has not been investigated extensively. This is partially due ot the difficulty in analyzing the damage at molecular level. This project intends to study the DNA repair of ionizing radiation damage by the following methods:
(1) Compare the killing effect and mutagenic effect of ionizing radiation on ataxia telangiectasia cells which are radiation sensitive.
(2) Search for the possible repair stimulating effect of human placenta extract.
(3) Epidemiological survey of the effect of low dose on human population.
2. Results and Discussion:
Ataxia telangiectasia cells were approximately 3 times as sensitive as the normal cells to ionizing radiation in killing effect, but were not more sensitive in mutagenesis by ionizing radiation than normal cells. This suggests that the high sensitivity of ataxia telangiectasia cells to ionizing radiation may not be due to the deficiency in DNA repair. Although human placenta extract was reported to be effective in protecting the killing effect of ionizing radiation in mice even when given after the exposure, we failed to confirm the effect. Consrquently, it is unlikely that the human placenta extract stimulate the repair of ionizing radiation damage. Epidemiological survey did not reveal the effect of low dose as the possible cause of malformation and Down's syndrome.
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