1986 Fiscal Year Final Research Report Summary
<^(19)F> NMR Study of Fluorinated Compounds in vivo with a view to Medical Application.
| Project/Area Number |
59460194
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Radiation science
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| Research Institution | Kyushu University |
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Principal Investigator |
KOJIMA Masaharu Kyushu University, Faculty of Pharmaceutical Sciences, 薬学部, 教授 (90037565)
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| Co-Investigator(Kenkyū-buntansha) |
MAEDA Minoru Kyushu University, Faculty of Pharmaceutical Sciences, 薬学部, 助教授 (70101178)
KAMEI-KANAZAWA Yoko Kyushu University, Faculty of Pharmaceutical Sciences, 薬学部, 助教授 (20037584)
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| Project Period (FY) |
1984 – 1986
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| Keywords | F-19 NMR / NMR Imaging of hetero nucleus / F-compound / In vivo Dynamics / Glucose metabolism / 2-Deoxy-2-fluoro-D-hexose / FDG / 麻酔剤 |
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| Research Abstract |
1. The purpose of this project is a basic in vivo biochemical study of fluorine compounds including fluorine containing pharmaceuticals with a view to medical application by the use of F-19 NMR.
2. We succeeded in the construction of a F-19 NMR chemical shift image with a phantom consists of inhalation aneshetics, halothane and methoxyflurane, adsorbed to the lipid dispersions as a model of brain.
3. We developed new, improved synthetic methods of fluorine compounds including 2-deoxy-2-fluoro-D-glucose(FDG) a pharmaceutical used for the monitoring of glucose utilization, 2-deoxy-2-fluoro-D-mannose(FDM), and 2-deoxy-2-fluoro-D-galactose.
4. The metabolic fate of FDG was studied in mice with excised organs by F-19 NMR. It was found that FDG injected to mice was converted to FDM(or FDM-6-phosphate) in vivo. The reaction was proved to be reversible by FDM injection, i.e., FDG is accumulated in tissues in the form of FDG-6-phos-phate and FDM-6-phosphate. The previous concept of FDG trapping mechanism at the site of high glucose metabolism was revised by this study.
5. The F-19 NMR images of FDG and FDGal were made from a slice of brain and a whole body of mice with the concentrations of fluorinated hexose of 2 - 3 mM or less. Although the contrast and the resolution of these images were not satisfactory at the present stage, the present result gives a strong promise to the future development of this method.
6. We hope to continue this project by the employment of F-19 imaging system at high magnetic field.
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