1986 Fiscal Year Final Research Report Summary
Transitions in Proteoglycan Types during Embryonic Cell Differentiation
| Project/Area Number |
59470129
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
物質生物化学
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| Research Institution | Nagoya University |
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Principal Investigator |
SUZUKI Sakaru Nagoya Univ., Faculty of Science, Professor, 理学部, 教授 (50022504)
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| Co-Investigator(Kenkyū-buntansha) |
HABUCHI Hiroko Nagoya Univ., Faculty of Science, Assistant, 理学部, 助手 (70109263)
TSUJI Masahiro Nagoya Univ., Faculty of Science, Assistant, 理学部, 助手 (80022739)
KIMATA Koji Nagoya Univ., Faculty of Science, Assistant, 理学部, 助手 (10022641)
NAKANISHI Yasuo Nagoya Univ., Faculty of Science, Assistant Professor, 理学部, 助教授 (40022636)
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| Project Period (FY) |
1984 – 1986
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| Keywords | proteoglycan / chondroitin sulfate / dermatan sulfate / chondrocyte / extracellular matrix / dermis / フィブロネクチン |
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| Research Abstract |
1. We have isolated and characterized a major proteoglycan component (PG-M) from stage 22-23 chick embryo limb buds. PG-M was larger in hydrodynamic size, richer in protein, and contained fewer chondroitin sulfate chains as compared to the cartilage proteoglycan (PG-H; Mr = 1.5 x <10^6> ). The core protein of PG-M was structurally different from the core protein of PG-H, as ascertained by tryptic peptide mapping. The expression of genetically distinct proteoglycan species may well be an important mechanism by which distinct morphogenesis are established.
2. We have demonstrated that the hyaluronate-binding activity of chick cellular fibronectin was due to a proteoglycan identical to PG-M. This proteoglycan also bound directly to type <I> collagen, suggesting that PG-M mediates interactions between hyaluronate, fibronectin, and type <I> collagen for matrix assembly.
3. We have found a striking change in relative concentrations of dermatan sulfate- and chondroitin sulfate-proteoglycan during the development of fetal rat skin, a process which may be involved in the development-related transition of extracellular matrix.
4. We have been able to correct the abnormal matrix formed by cmd/cmd chondrocytes by adding PG-H to the culture medium.
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