1986 Fiscal Year Final Research Report Summary
Analysi of molecular mechanisms of tissue reparative regeneration through transdifferentiation
| Project/Area Number |
59480023
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
動物発生・生理学
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| Research Institution | National Institute for Basic Biology, Okazaki National Research Institutes |
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Principal Investigator |
EGUCHI Goro Professor, National Institute for Basic Biology, Okazaki National Research Institutes, その他, 教授 (80022581)
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| Co-Investigator(Kenkyū-buntansha) |
HAMADA Yoshio Research Associate, National Institute for Basic Biology, Okazaki National Resea, 基礎生物学研究所, 助手 (10132739)
AGATA Kiyokazu Research Associate, National Institute for Basic Biology, Okazaki National Resea, 基礎生物学研究所, 助手 (70167831)
KODAMA Ryuji Research Associate, National Institute for Basic Biology, Okazaki National Resea, 基礎生物学研究所, 助手 (90161950)
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| Project Period (FY) |
1984 – 1986
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| Keywords | Reparative regeneration / Transdifferentiation / Dedifferentiation / Redifferentiation / Gene expression / 遺伝子調節 |
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| Research Abstract |
1) We established a unique cell culture system of chick embryonic pigmented epithelial cells (PECs) for studying cellular and molecular mechanisms of transdifferentiation. By this system a pure and large population of multipotent dedifferentiated cells (dePECs) can be produced and each process of transdifferentiation from PECs to lens phenotypes can be artificially regulated. The bipotent nature of these dePECs for differentiating to either lens or pigment cells was clearly demonstrated.
2) Using this system we characterized the nature of multipotent dePECs and demonstrated that these dePECs maintained their bipotent nature differentiating to either lens or pigment cells through about 15 mitotic cycles and then gradually lost their potential for differentiating pigment cell phenotypes. The mode of expressin of differentiative potentials in dePECs must be closely related to their aging.
3) We succeeded in production of monoclonal antibodies against newt lens specific crystallins. Applying these antibodies to scleen cDNA library, we cloned a gene for the major enbryonic <gamma> -crystallin subclass (named <gamma> A1) and determined its whole base sequence. It was demonstrated that the gene for <gamma> A1 was specifically expressed in newt lens cells but not in pigmented epithelial cells of the normal adult newt iris.
4) We succeeded in production of unique monoclonal antibodies crossreacting with newt cell surface or intercellular molecules which widely distributed in many types of newt tissues including dorsal and ventral iris epithelia. The antigen molecules was found to decay rapidly in only the dorsal marginal iris, from which a lens was regenerated, soon after lentectomy, suggesting the essential roles of these antigen molecules in regulation of regeneration.
These results obtained through this General Research should be the fundamental information for deeply understanding the regulatory mechanism of regeneration.
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