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1986 Fiscal Year Final Research Report Summary

Pathogenetic relationship between spontaneous developments of thymoma and muscle abnormality in Buffalo/Mna rats.

Research Project

Project/Area Number 59480155
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionAichi Cancer Center

Principal Investigator

MATSUYAMA Mutsushi  Aichi Cancer Center Research Institute, Chief, その他, その他 (80073112)

Co-Investigator(Kenkyū-buntansha) WATANABE Minoru  Nagoya City University of Pharmacy School, Professor, 藥学部・藥品作用学教室, 教授 (50012638)
NISHI Yoshimi  Aichi Cancer Center Research Institute, Research Fellow, 研究所・超微形態学部, 研究員 (50073101)
MASUDA Akira  Aichi Cancer Center Research Institute, Research Fellow, 研究所・超微形態学部, 研究員 (50157202)
Project Period (FY) 1984 – 1986
KeywordsRat of the Buffalo / Mna strain / Spontaneous thymoma / Dominant inheritance / Atrophy of fast twitch muscles / Effects of the introduction of athymic nude gene / Effects of neonatal thymectomy / Epithelial cell lines of rat thymus and thymoma / 培養液へのデキサメサゾン添加効果
Research Abstract

We found previously that Buffalo/Mna rats developed spontaneous thymoma, muscle atrophy and glomerulosclerosis at nearly 100%. The present genetic study using crosses between Buffalo/Mna and ACI rats has shown that thymoma susceptibility was determined by a single autosomal dominant gene Tbm-1. The relationships between developments of thymoma, muscle atrophy and glomerulosclerosis were analysed, by using of neonatally thymectomized Buffalo/Mna rats or Buffalo/Mna-rnu/+ rats introduced with athymic nude gene of the rat (rnu). Three day thymectomy produced no effect on the developments of muscle strophy and glomerulosclerosis. In heterozygous Buffalo/Mna-rnu/+ rats, the developments of thymoma and glomerulosclerosis were extremely inhibited, but the development of muscle atrophy was not, compared to littermate Buffalo/Mna-+/+ rats. These results reveal that muscle atrophy of Buffalo/Mna rats is not an associated syndrome with thymoma development, but it is a completely independent abnor … More mality. The inhibitory mechanisms of rnu gene against the thymoma development and the linkage between the developments of thymoma and glomerulosclerosis will be the subjects for future studies.
Cell biological examinations have been performed to elucidate the nature of thymoma. Thymoma epithelial cells of primary cultures had larger cytoplasm, about 2 times as large as thymus epihtelial cells, and secreted a polypeptide of molecular weight about 10,000, which can induce non-specific proliferation of thymic lymphocytes, showing a characteristic of functioning tumor cells. Long-term cultures of thymus and thymoma epithelial cells have been difficult, but we have found that these epithelial cells can proliferate steadily, showing a polygonal shape, in a medium containing 1 <maicro> M dexamethasone. Six thymus and 6 thymoma epithelial cell lines were established as clonal cell lines. We will perform molecular biological analyses on thymoma, using these cell lines and cooperating with molecular biologists. Less

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Akira Masuda: Virchows Archiv.[Cell Pathol.]. 46. 283-296 (1984)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akira Masuda: Exp.Cell Res.160. 343-355 (1985)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takamichi Nakamura: Nephron. 43. 50-55 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mutsushi Matsuyama: Jpn.J.Cancer Res.(Gann). 77. 1066-1068 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mutsushi Matsuyama: Jpn.J.Cancer Res.(Gann). 78. 40-44 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tateru Toyokawa: Cell.Mol.Biol.January. (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akira Masuda: "Cytological characteristics of cultured epithelial cells from thymomas in BUF/Mna rat." Virchows Archiv. [Cell Pathol.]. 46. 283-296 (1984)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akira Masuda: "Dexamethasone-induced changes in morphology and keratin organization of rat thymic epithelial cells in primary culture." Exp. Xell Res.160. 343-355 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takamichi Nakamura: "Sclerotic lesions in the glomeruli of Buffalo/Mna rats." Nephron. 43. 50-55 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mutsushi Matsuyama: "A single dominant susceptible gene determines spontaneous development of thymoma in BUF/Mna rat." Jpn. J. Cancer Res. (Gann). 77. 1066-1068 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mutsushi Matsuyama: "Possible single dosege effects of the nude gene: Suppression of spontaneous development of thymoma and nephropathy in BUF/Mna-run/+ rats." Jpn. J. Cancer Res. (Gann). 78. 40-44 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tateru Toyokawa: "Remarkable changes in mitogen responsiveness and rosette-forming capacity of thymocytes with aging due to possible disorders of thymic hormone secretion from thymus epithelial cells of spontaneous thymoma Buffalo/Mna rats." Cell. Molec. Biol.January (1987)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1988-11-10  

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