1986 Fiscal Year Final Research Report Summary
On the protein synthesis in the undifferentiated cells by mesodermal inducing substances.
| Project/Area Number |
59540467
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
動物発生・生理学
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| Research Institution | Yokohama City University, Department of Biology Lib. abd Arts Fac., |
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Principal Investigator |
ASASHIMA Makoto Yokohama City University, ( Professor )., 文理学部, 教授 (00090564)
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| Project Period (FY) |
1984 – 1986
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| Keywords | Mesodermal induction / Inducing substances / Inducer protein / Differentiation Amphibian embryo / Muscle differentiation / 分化 / 蛍光抗体法 / 筋肉分化 |
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| Research Abstract |
Mesodermal indicing substances from carp swim bladder were purified by biochemical methods such as affinity with Con.A Sepharose and gel electrophoresis. We obtained highly active inducing fractions " F1 " by affinity with Con. A Sepharose and "G1" and "G4" by gel electrophoresis. The substances containing these fractions induced the presumptive ectoderm in mesoderma; tissues such as muscle and notochord in over 60% of the histological sections. "G1" and "G4" showee one or two bands with Coomassie staining. We attempted the two-dimensional polyacrylamide gel electrophoresis to see if F1 inducer could induce or activate protein synthsis in the presumptive ectoderm. In the explants with F1 inducer after two weeks' culture, more than 82 protein spots were observed on the gel. Comparison with control experiments showed that our inducing substances induce some new proteins in the explants. Immunochemical studies of muscle differentiation by mesodermal inducing substances were also examined at the molecular level.
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