1986 Fiscal Year Final Research Report Summary
ヒト胚細胞性腫瘍の分化関連糖鎖抗原の免疫学的並びに生化学的研究と臨床診断への応用
| Project/Area Number |
59570699
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Chiba University |
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Principal Investigator |
SEKIYA Souei Chiba University, School of Medicine, Dept of Obstetrics and Gynecology, 医学部, 講師 (00092065)
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| Co-Investigator(Kenkyū-buntansha) |
KAWATA Makoto Kawatetsu Chiba hospital, Dept of Obstetrics and Gynecology, 産婦人科, 部長
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| Project Period (FY) |
1984 – 1986
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| Keywords | Germcell tumor / Glycopeptide / lectin / Carbohydrate antigen / 分化抗原 |
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| Research Abstract |
Human antibody against an embryoglycan present on a mouse teratocarcinoma cell line F9 was found in sera from 16 out of 29 patients with embryonal carcinoma, yolk sac tumor, immature teratoma, and choriocarcinoma of gonadal and extragonadal origins by Farr assay. In contrast, none of sera from patients (77 cases) with dysgerminoma, seminoma, germinoma, and mature teratoma, or patients (118 cases) with non-germ cell types of ovarian tumors contained this antibody. The antigenic embryoglycan was of high molecular weight (more than 70,000) on Sephacryl S300 column chromatography, and carried binding sites for Grifonia simplicifolia agglutinin-1. The antigenic embryoglycan was also found in F9 cell-cultured medium. Absorption of patients' sera with synthetic blood group B trisaccharides failed to remove the antibody against F9 embryoglycan. None of these patients' sera showed higher hemaggulutination titer to rabbit erythrocytes than the normal range. In contrast, <alpha> -galactosyl carbohydrates obtained from Ehrlich ascites tumor cell effectively inhibited the binding of patients' sera with F9 embryoglycan. These results indicate that the human antibody against F9 embryoglycan recognizes <alpha> -galactosyl structures, that are distinct from B blood group antigen, but are cross-reactive with <alpha> -galactosyl structures on Ehrlich ascites cells.
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Research Products
(10 results)
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[Publications] Sekiya,S;,Kawata,M;,Iwasawa,H;,Inaba,N;,Sugita,M;,Suzuki,N;,Motoyama,T;,Yanamoto,T.;,: Takamizawa,H.Differentiation. 29. 259-267 (1985)
Description
「研究成果報告書概要(和文)」より
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[Publications] Sekiya,S;,Tomita,Y;,Chen,H-Y;,Kawata,M;,Oosaki,T;,Kuwata,T;,Takamizawa,H.: Differentiation.
Description
「研究成果報告書概要(和文)」より
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[Publications] Sekiya,S., Kawata,M., Iwasawa,H., Inaba,N., Sugita,M., Suzuki,N., Motoyama,T., and Takamizawa,H.: "Characterization of human embryonal carcinoma cell line derived from testicular germ-cell tumors." Differentiation. 29. 259-267 (1985)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Sekiya,S., Tomita,Y., Chen,H-Y., Kawata,M., Oosaki,T., Kuwata,T., and Takamizawa,H.: "Sensitivity of human germ-cell-tumor cell lines to human interferons." Differentiation. (1987)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Sekiya,S., Ishige,H.,Yamazawa,K.,Kawata,M., Inaba,N., Nagao,K., and Takamizawa,H.: "The capacity for differentiation and production of human chorionic gonadotropin and <alpha> -fetoprotein in human embryonal carcinoma cell lines."
Description
「研究成果報告書概要(欧文)」より
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[Publications] Kawata,M., Higaki,K., Sekiya,S., and Takamizawa,H.: Noguchi,Y., and Muramatsu,T.(editor), Rikoh-Gakusha. Molocular analysis of cell surface antigens on human germ cell tumors, 6-1 - 6-26 (1987)
Description
「研究成果報告書概要(欧文)」より
