1986 Fiscal Year Final Research Report Summary
Basic studies on development of the automatic cell detection system using voltammetry
| Project/Area Number |
59850134
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
工業物理化学
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
MATSUNAGA Tadashi Tokyo University of Agriculture and Technology, Associate Professor, 工学部, 助教授 (10134834)
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| Co-Investigator(Kenkyū-buntansha) |
OSUMI Masako Japan Women's University, Professor, 家政学部, 教授 (60060646)
中島 幸一 福岡歯科大学, 口腔衛生学, 助教授
NAKASHIMA Koichi Fukuoka Dental College, Associate Professor
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| Project Period (FY) |
1984 – 1986
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| Keywords | Classification of microbial cells / Cyclic voltammogram / 4.4'-bipyridine / 抗生物質耐性 / 尿路感染症 |
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| Research Abstract |
Electrodesystems were developed for the detection and classification of microbial cells and animal cells. Cyclic voltammograms were obtained with a basal plane pyrolytic graphite electrode used alone or modified with 4,4'-bipyridine. Electron transfer between cells and the electrode was mediated by coenzyme A present in the cell wall. The number of various microbial cells was determined from the peak current of cyclic voltammograms. Intenstinal bacteria were classified as gram-positive or gram-negative by the system. Gram-positive bacteria gave peak currents at 0.65 to 0.69V versus the saturated calomel electrode (SCE). The peak potentials of gram-negative bacteria were 0.70 to 0.74V versus SCE. Gram-negative and gram-positive bacteria were also classified based on the ratio of the second peak current to be first peak current when the potential sweep was repeated twice.
Wild and antibiotic resistant cells were also classified with the electrode system. Microbial cells were incubted at 3
… More
7 C for 1h in the medium containing antibiotics, and cyclic voltammograms were obtained in the range of 0 to 1.0V versus SCE. The peak current of wild strains decreased or constant whereas the peak current of antibiotic resistant strains increased.
The system was also applied to diagnosis of urinary tract infection and antibiotic selection. Before measurements, viable cell numbers were manipulated by incubating urine containing bacteria with Rogosa medium for 3h. The peak current of cyclic voltammogram increased with increasing the initial cell concentration of E.coli in urine. Susceptability of bacteria to various antibiotics was also determined from the peak current when they were incubated for 3h in the mixture of urine and Rogosa medium.
Animal cells such as T cells, B cells, macrophage and tumor cells were detected by the system. The system was also applied to the cell classification coupled with antigen-antibody reactions. Thus, the system was shown to be applicable in the medical and industrial fields. Less
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