1986 Fiscal Year Final Research Report Summary
Ultrahigh speed quantitative autoradiography
| Project/Area Number |
59870007
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
TASAKI Kyoji Tohoku University School of Medicine, 医学部, 教授 (10004532)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAYE Toshio Tohoku University School of Medicine, 医学部, 助手 (20155659)
SUZUKI Hitoshi Tohoku University School of Medicine, 医学部, 講師 (60004727)
TSUKAHARA Yasuo Research Center for Applied Information Sciences, 応用情報学研究センター, 教授 (60004587)
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| Project Period (FY) |
1983 – 1986
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| Keywords | rapid autoradiography / rapid ARG / high speed ARG / quantitative ARG / 画像処理 |
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| Research Abstract |
The present study has been undertaken for the purpose of developing a new system of quantitative autoradiography, which is provided with the merit of film-based conyentional autoradiography (two-dimentional spatial information), together with the merit of high sensitivity liquid scintillation counting (very high speed). The system we have developed consists of a thin film scintillator,which acts to convert beta radiation to photons, and a real time two-dimentional low light level imaging system (Hamamatsu Photonics C1966).
1) Scintillator Three kinds of scintillators, plastic, liquid and a spray type autoradiographic enhancer, were tested. The plastic scintillator is least sensitive to radiation, but still suitable for 18-F and 14-C because of its easy handling. Although the liquid and spray enhancer are used for all three radionuclides used in the present study, 18-F, 14-C and 3-H, a low background liquid scintillator was found to be most suitable for the beta emitter of low energy such as 3-H.
2) Rapid autoradiography When the lowest energy beta emitter, 3-H, is combined with the liquid scintillator, the exposure time can be shortened upto 10 min to obtain a well visible image at a low concentration of 265 Bq/ <mm^2> . For 14-C and 18-F one min is more than sufficient with any of the three scintillator used.
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