1990 Fiscal Year Final Research Report Summary
Automated Direct Assay System for The Measurement of Sex Steroid Hormones in Serum Using High Performance Liquid Chromatog
| Project/Area Number |
59870054
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Okayama University |
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Principal Investigator |
SEKIBA Kaoru Okayama University, Medical School, Professor, 医学部, 教授 (90033042)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMIZU Kenji Okayama University, Medical School Hospital, Resident Doctor, 医学部附属病院, 医員
URABE Kiyoshi Okayama University, Medical School Hospital, Assistant Prof., 医学部附属病院, 助手 (70127546)
YASHIDA Nobutaka Okayama University, Medical School Hospital, Assistant Prof., 医学部附属病院, 助手 (80127597)
HAYASHI Nobuyoshi Okayama University, Medical School Hospital, Assistant Prof., 医学部附属病院, 助手 (10164948)
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| Project Period (FY) |
1984 – 1986
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| Keywords | High Performance Liquid Chromatography / Automated Analyzing System / Sex Sterois Hormone / Estradiol / Progesterone / Serum Concentration / Electrochemical Detection / Ultraviolet Spectrophotometric Detection |
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| Research Abstract |
An automated direct assay system using high performance liquid chromatography with electrochemical detection and ultraviolet spectrophotometric detection was developed for the simultanltaneous measurement of estradiol, estrone, progesterone, 17alpha-hydroxyprogesterone, 20alpha-hydroxyprogesterone, testosterone and androstenedione in biological fluids. It takes 30 min to measure estrogens, and 80 min to measure whole steroid hormones. The recovery rates of steroid hormones were 77-84%, and the intra-assay coefficients of variation of the peak height were 1.7-3.1%. The relationship between the dose of each steroid hormone and the peak height showed good linearities. A comparison between the values measured by this method and by radioimmunoassay revealed good correlation for estradiol (r=0.938, p<0.001) and progesterone (r=0.903, p<0.001). Estradiol and estrone could be analysed above the level of 250 pg/ml, andprogesterone, 17alpha-hydroxyprogesterone, 20alpha-hydroxyprogesterone, testosterone and androstenedione could be analyzed above the level of 5.0-7.5 ng/ml. The method was applied to the clinical appraisal of placental function and maturation of ovarian follicles.
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