1987 Fiscal Year Final Research Report Summary
Biochemical srudies on the roles of prostaglandins, leukotrienes and PAf in pathogenesis
| Project/Area Number |
60304048
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Tokyo Medical and Dental University (1986-1987)
Tokyo Metropolitan Institute of Gerontology (1985) |
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Principal Investigator |
MUROTA Sei-itsu Tokyo Medical and Dental University, Faculty of Dentistry, Professor, 歯学部, 教授 (50072989)
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| Co-Investigator(Kenkyū-buntansha) |
TOMIOKA Hisao Chiba University, School of Medicine, Associate Professor, 医学部, 助教授 (20009632)
YAMAMOTO Shozo Tokushima University, School of Medicine, Professor, 医学部, 教授 (50025607)
NOJIMA Shoshichi Teikyo University, Faculty of Pharmaceutical Sciences, Professor, 薬学部, 教授 (70090470)
SHIMIZU Takao University of Tokyo, Faculty of Medicine, Associate Professor, 医学部, 助教授 (80127092)
KATORI Makato Kitasato University, School of Medicine, Professor, 医学部, 教授 (50050365)
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| Project Period (FY) |
1985 – 1987
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| Keywords | Prostaglandin / Leukotriene / PAF |
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| Research Abstract |
New findings of this research project are as follows; 1. Kallikrein was found to stimulate prostacyclin production in endothelial cells in culture. Regarding this phenomena, it was disclosed that kallikrein could produce a new peptide which could stimulate phospholipase A_2 of endothelial cells in a significant way. (S.Murota) 2. Platelet-activating factor was found to be able to stimulate metabolism phosphoinositides through phospholipase A_2 in primary cultured rat hepatocytes. (T.Ishibashi) 3. PGD_2 binding to rabbit platelets was found to increase by sbout 150% in the presence of isoproterenol. It was suggested that a B_2-adrenoceptor-mediated cAMP-dependent mechanism exists for the regulation of PGD_2 receptor binding in rabbit platelets. (A.Ichikawa) 4. Leukotoxin, a linoleate epoxide, was discovered from human burned skin and it was found that leukotoxin showed a typical uncoupling effect on mitchondrial respiration. (T.Ozawa) 5. Using 2 different rat allergic inflammatory models: IgE-mediated and IgE-antigen complex-induced pleurisies, roles of leukotrienes in inflammation was studied. (M.Karoti) 6. Molecular cloning of a cDNA coding for human LTA_4 hydrolase was achieved. (T.Shimuzu) 7. It was suggested that protein kinase C served as an inhibitor for PGE_1-induced Ca^<2+> influx in Swiss 3T3 cells. (Y.Takai) 8.
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