| Project/Area Number |
60304053
|
|---|
| Research Category |
Grant-in-Aid for Co-operative Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Virology
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
NAGAI Yoshiyuki Nagoya University School of Medicine, Professor, 医学部, 教授 (20022874)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HOMMA Morio Kobe University School of Medicine, Professor, 医学部, 教授 (10004566)
HOSAKA Yasuhiro Research Institute for Microbial Diseases, Osaka Univ. As.Prof., 微生物病研究所, 助教授 (50029766)
ITO Yasuhiko Mie University School of Medicine, Professor, 医学部, 教授 (00022872)
YAMANOUCHI Kazuya Institute of Medical Science, University of Tokyo, Professor, 医科学研究所, 教授 (30072888)
SHIBUTA Hiroshi Institute of Medical Science, Universoty of Tokyo, Professor, 医科学研究所, 教授 (70012721)
|
|---|
| Project Period (FY) |
1985 – 1987
|
| Keywords | Paramyxovirus / Genome structure / Biosynthesis / Spread in hosts / Pathogenicity / 抗原性 |
|---|
| Research Abstract |
The aim of this research has been to gain knowledges on the molecular biology and pathogenesis of a virus family, Paramyxoviridae. The primary structure of the entire genome of about 15,000 nucleotides was determined for Sendai virus and type 3 parainfluenza virus. With the latter, the gene products affecting the viral pathogenicity were identified. The information on teh genome, which plays key roles in virus spread and pathogenesis in infected hosts, was identified for Newcastle disease virus (NDV) by comparing the glycoprotein gene sequences between virulent and avirulent strains. The specificity of cellular protease(s) playing a key role in Sendai virus pathogenesis in murine respiratory tract, was identified by sequencing various variants showing different protease sensitivity. The amino acids participating in antigenic sites were identified for NDV by sequencing the variantg escaping neutralization by each monoclonal antibody. The system to analyze cellular immune response was establighed for mumps virus. Basic cell biological analyses were done in paramyxovirus infected cells. These provided such new information that cellular cytoskeleton is involved in virus assembly. In summary, this study opened a new aspect of virus research, namely, the combination or fusion of viral molecular biology and pathogenesis. We hope that this new trend in virus research will progress further and provide novel concepts for control of virus diseases.
|
