1988 Fiscal Year Final Research Report Summary
Multidiciplinary approach to the clinical pancreas and islet transplantation
| Project/Area Number |
60304067
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | University of Tokyo, Faculty of Medicine |
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Principal Investigator |
IDEZUKI Yasuo University of Tokyo, Faculty of Medicine, 医学部, 教授 (60081717)
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| Co-Investigator(Kenkyū-buntansha) |
UMEYAMA Kaoru Osaka Municipal University, Faculty of Medicine, 医学部, 教授 (30046792)
NAKAGAWARA Gizo Fukui Medical College, 医学部, 教授 (10019549)
IWASAKI Yoji Tsukuba University, Faculty of Medicine, 臨床医学系, 教授 (30009112)
TSUCHIYA Ryoichi Nagasaki University, Faculty of Medicine, 医学部, 教授 (70025534)
MITO Michio Asahikawa Medical College, 医学部, 教授 (60000981)
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| Project Period (FY) |
1985 – 1986
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| Keywords | Pancreatic transplantation / ラ島移植 / インスリン依存型糖尿病 / 臓器移植 / 人工膵 / 免疫抑制 |
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| Research Abstract |
Problems in pancreatic and islet transplantation have been studied in animal experiments and in limited number of clinical trials. Pancreatic transplantation was performed experimentally in dogs and in rats using several techniques (pan-creaticoduodenal transplantation, whole pancreas transplantation, segmental transplantation, pancreas transplantation with duodenal cuff, etc.). Long-term survival (more than 18 months) of dogs with a functioning pancreatic allograft was obtained using the immunosuppression regimen using ciclosporin and imuran. Cytotoxic effect of ciclosporin on beta celles was dose-depandent and the damage caused by the drug was reversible. Immunosuppresive effect of Mizoribine was weak and could not significantly extend the survival of the allograft. Develop-ment of microangiopathies due to diabetes mellitus was prevented or reversed by pancreatic transplantation when the transplantation was performed in the early stage of microangiopathy. These results are encouraging for the clinical appli-cation of pancreatic transplantation.
Possibility of islet transplantation was studied in animals. New technique of islet seperation by perfusion with a collagenase containing solution or freezing seems promising. Survival of islet xenograft was extended by ciclosporin and radiation, but further study and refinement of the technique is necessary before clinical application of this new technique. Encapsulation of the islets by an artificial membrane is possible and this may be used as an implantable artificial pancreas. the hybrid artificial pancreas secreted insulin after implantation into the abdominal cavity for more than 1 week and without immunosuppression. Finally, national survey of the clinical application of the pancreatic and islet transplantation revealed that 1 pancreaticoduodenal allotransplantation, 10 auto-transplantation of the segmental pancreas, and 12 islet autotransplantation have been performed clinically in Japan by October, 1986.
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