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1988 Fiscal Year Final Research Report Summary

Organellar DNA variation in some economic plants and their wild relatives

Research Project

Project/Area Number 60400005
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field 広領域
Research InstitutionKyoto University

Principal Investigator

TSUNEWAKI Koichiro  Faculty of Agriculture,Kyoto University, 農学部, 教授 (20026438)

Project Period (FY) 1985 – 1988
KeywordsOrigin of cultivated species / Phylogeny / Organellar DNA / Restriction endonuclease analysis / RFLP analysis / Cytoplasm donor / Gramineae / tuber crops(根菜類)
Research Abstract

The present project has aimed to analyze organellar DNA variation in economic plants and their wild relatives, in order to clarify their phylogenetic relationships as well as the origin of the former. The materials used, the methods employed and the results obtained are as follows:
(1) From 10 cereal species, representing 7 tribes in Gramineae, chloroplast (ct) DNAs were extracted and their restriction endonuclease analysis was performed. A phylogenetic tree constructed for their chlorplast genomes was the same as that previously established for those species by taxonomical studies, except one minor point.
(2) From practically all the species of two related genera Triticum (wheat) and Aegilops, ct and mt (mitochondrial) DNAs were extracted, and studied. With their ctDNAs, restriction endonuclease analysis, construction of physical maps and complete sequencing of a gene rbcL for the Rubisco's large subunit were carried out, whereas restriction endonuclease analysis and RFLP analysis were … More made with mtDNAs. From those results, phylogenetic relationships among chloroplast and mitochondrial genomes were clarified, and the maternal parents of almost all polyploid species were determined.
(3) From 5 species of Secale (fye), 7 species and 17 subspecies of Hordeum (barley), and 19 species of Avena (oats), ctDNAs were extracted, and physical map construction and restriction endonuclease analysis were made. From these results, interspecific relationships in each genus, and the origin of crop species were revealed. The rates of evolutionary changes of ctDNAs in 5 genera, Triticum, Aegilops, Secale, Hordeum and Avena, appeared to be almost the same.
(4) Using 4 A-genome diploid species (66 accessions) of Oryza (rice), restriction endonuclease analysis of ctDNA was carried out. From the results, the origin of cytoplasms of 2 cultivated species and 2 ecospecies, Indica and Japonica, was elucidated.
(5) Using 13 species and 22 varieties of a bamboo genus Phy11ostachys, restriction endonuclease analysis and RFLP analysis of mitochondrial DNA were made. Based on the results, a phylogenetic tree of these species was constructed.
(6) Using 4 species (15 clones) of a yam genus Dioscrea, and 20 species (44 clones) of Solanum, restriction endonuclease analysis of ctDNA was carried out. From the results, interspecific relationships in each genus and the origin of cultivated species were clarified.
(7) Restriction endonuclease analysis of ctDNAs from 11 Prunus species was made, and their phylogenetic relationships were clarified. The female parent of Someiyoshino was found to be Edohigan. Less

  • Research Products

    (48 results)

All Other

All Publications (48 results)

  • [Publications] Enomoto,S.: Japanese Journal of Genetics. 60. 411-424 (1985)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kaneko,T.: Japanese Journal of Genetics. 61. 157-168 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kataoka,J.: Barley Genetics. 5. 515-524 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Murai,K.: Genetics. 116. 613-621 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ogihara,Y.: Theoretical and Applied Genetics. 76. 321-332 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishii,T.: Japanese Journal of Genetics. 63. 523-536 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hosaka,K.: Theoretical and Applied Genetics. 72. 606-618 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ikeda,T.M.: Proceedings of 7th International Wheat Genetics Symposium,Cambridge. 751-756 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishii,T.: Japanese Journal of Genetic. 61. 537-541 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mori,N.: Proceedings of 7th International Wheat Genetics Symposium,Cambridge. 109-114 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Murai,K.: Heredity. 57. 335-339 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Murai,K.: Proceedings of 2nd International Oats Conference,Aberystwith. 34-38 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] murai,K.: Japanese Journal of Genetics. 64. 35-47 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ogihara,Y.: Proceedings of 7th International Wheat Genetics Symposium,Cambridge.127-132 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Siregar,U.J.: Proceedings of 7th International Wheat Genetics Symposium,Cambridge. 145-151 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Taguchi,F.: Bamboo Journal(Japan). 6. (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Taguchi,F.: Bamboo Research(China). in press.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Terachi,T.: Theoretical and Applied Genetics. 73. 175-181 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Terachi,T.: Japanese Journal of Genetics. 62. 375-387 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Terachi,T.: Proceedings of 7th International Wheat Genetics Symposium,Cambridge. 789-795 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Terauchi,R.: Theoretical and Applied Genetics. 77. (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsunewaki,K.: Proceedings of 7th International Wheat Genetics Symposium,Cambridge. 53-62 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsunewaki,K.: Genome. 31. (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Enomoto,S.: "Studies on the origin of crop species by restriction endonuclease analysis of organellar DNA. I. Phylogenetic relationships among ten cereals revealed by the restriction fragment patterns of chloroplast DNA" Japanese Journal of Genetics. 60. 411-424 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hosaka,K.: "Who is mother of the potato? -- restriction endonuclease analysis of chloroplast DNA of cultivated potatoes" Theoretical and Applied Genetics. 72. 606-618 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ikeda,T.M.: "Biochemical studies on chlorophyll deficiency induced in common wheat by a C^u cytoplasm" Proceedings of 7th International Wheat Genetics Symposium, Cambridge. 751-756 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishii,T.: "Restriction endonuclease analysis of chloroplast DNA from cultivated rice species, Oryza sativa and O. glaberrima" Japanese Journal of Genetics. 61. 537-541 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishii,T.: "Restriction endonuclease analysis of chloroplast DNA from A-genome diploid species of rice" Japanese Journal of Genetics. 63. 523-536 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kaneko,T.: "Studies on the origin of crop species by restriction endonuclease analysis of organellar DNA. II. Restriction analysis of ctDNA of 11 Prunus species" Japanese Journal of Genetics. 61. 157-168 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kataoka,J.: "Chloroplast DNA variation observed among Hordeum species" Barley Genetics. 5. 515-524 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mori,N.: "Organellar genome differentiation in wild tetraploid wheats, Triticum dicoccoides and T. araraticum" Proceedings of 7th International Wheat Genetics Symposium, Cambridge. 109-114 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Murai,K.: "Molecular basis of genetic diversity among cytoplasms of Triticum and Aegilops species. IV. CtDNA variation in Ae. triuncialis" Heredity. 57. 335-339 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Murai,K.: "Phylogenetic relationships between Avena species revealed by the restriction endonuclease analysis of chloroplast and mitochondrial DNAs" Proceedings of 2nd International Oats Conference, Aberystwith. 34-38 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Murai,K.: "Chloroplast genome evolution in the genus Avena" Genetics. 116. 613-621 (116)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Murai,K.: "Studies on the origin of crop species by restriction endonuclease analysis of organellar DNA. III. Chloroplast DNA variation and interspecific relationships in the genus Secale" Japanese Journal of Genetics. 64. 35-47 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ogihara,Y.: "Diversity and evolution of chloroplast DNA in Triticum and Aegilops as revealed by restriction fragment analysis" Theoretical and Applied Genetics. 76. 321-332 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ogihara,Y.: "Chloroplast DNA diversity and evolution in Triticum and Aegilops" Proceedings of 7th International Wheat Genetics Symposium, Cambridge. 127-132 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Siregar,U.J.: "Aegilops searsii is a possible cytoplasm donor to Ae. kotschyi and Ae. variabilis" Proceedings of 7th International Wheat Genetics Symposium, Cambridge. 145-151 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Taguchi,F.: "Intra-and interspecific variation of mitochondrial DNA in three Phyllostachys species (In Japanese with English summary)" Bamboo Journal. 6. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Taguchi,F.: "Phylogenetic relationship between four Phyllostachys species revealed by restriction endonuclease analysis of their mitochondrial DNA" Bamboo Research. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Terachi,T.: "The molecular basis of genetic diversity among cytoplasms of Triticum and Aegilops. V. Mitochondrial genome diversity among Aegilops species having identical chloroplast genomes" Theoretical and Applied Genetics. 73. 175-181 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Terachi,T.: "The molecular basis of genetic diversity among cytoplasms of Triticum and Aegilops. VI. Complete nucleotide sequences of the rbcL genes encoding H- and L-type Rubisco large subunits in common wheat and Ae. crassa 4x" Japanese Journal of Genetics. 62. 375-387 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Terachi,T.: "The rbcL genes in wheat and several Aegilops species with divergent chloroplast genomes" Proceedings of 7th International Wheat Genetics Symposium, Cambridge. 789-795 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Taguchi,F.: "Intra- and interspecific variation of mitochondrial DNA in three Phyllostachys species" Bamboo Journal. 6. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Taguchi,F.: "Phylogenetic relationship between four Phyllostachys species revealed by restriction endonuclease analysis of their mitochondrial DNA" Bamboo Research.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Terachi,T.: "The molecular basis of genetic diversity among cytoplasms of Triticum and Aegilops. V. Mitochondrial genome diversity among Aegilops species having identical chloroplast genomes" Theoretical and Applied Genetics. 73. 175-181 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Terachi,T.: "The molecular basis of genetic diversity among cytoplasms of Triticum and Aegilops. VI. Complete nucleotide sequences of the rbcL genes encoding H- and L-type Rubisco large subunits in common wheat and Ae. crassa 4x" Japanese Journal of Genetics. 62. 375-387 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Terachi,T.: "The rbcL genes in wheat and several Aegilops species with divergent chloroplast genomes" Proceedings of 7th International Wheat Genetics Symposium, Cambridge. 789-795 (1988)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1990-03-20  

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