• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1987 Fiscal Year Final Research Report Summary

Medicolegal studies on severely burned bodies.

Research Project

Project/Area Number 60440041
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field Legal medicine
Research InstitutionKanazawa University

Principal Investigator

NAGANO Taizo  Department of Legel Medicine, Kanazawa University School of Medicine, 医学部法医学講座, 教授 (20073679)

Co-Investigator(Kenkyū-buntansha) TAKAYASU Tatsunori  Depaerment of Legal Medicine, Kanazawa University School of Medicine, 医学部法医学講, 助手 (80154912)
OHSHIMA Tohru  Department of Legal Medicine, Kanazawa University School of Medicine, 医学部法医学講座, 講師 (40183024)
MAEDA Hitoshi  Department of Legal Medicine, Kanazawa University School of Medicine, 医学部法医学講座, 助教授 (20135049)
TANAKA Noriyuki  Department of Legal Medicine, Kanazawa University School of Medicine (at present, 法医学講座, 教授 (60126597)
TSUJI Tsutomu  Department of Forensic Science, Saga Medical School, 法医学講座, 教授 (50073680)
Project Period (FY) 1985 – 1987
KeywordsBlood group antigen / Immunoelectronmicroscopy / Ultrastructure / Thermo-change / Forensic toxicology / volatile poison / CNS-active drug / GC-MS
Research Abstract

This research has been carried out in order to offer solutions of the following medicolegal problems concerning severely charred bodies; personal identification and diagnosis of cause of death.
1. Thermo-changes of human tissue cells and their blood group activities:
All the activities examined in the systems such as the ABO, Lewis, MNSs and Rh remained on the denaturated red cells after heating at 100゜c. The ABO and Lewis group activities were detectable after heating at 180-200゜c. Electron-microscopically, the thermodenaturated cells (60-100゜c) showed swelling and fragmentation of the membranes. By immunoferritin staining, the ABO group activities were demonstrated on the membranerelated substances which showed a coagulative change.
Ultrastructural localization of the ABO group activities in some kinds of cells such as mucous cells and endothelial cells of the vessels were shown by immunogold method. Nearly charred layuers of tissues were micro- and ultramicroscopically almost amorphous … More . Inner parts, retaining the microscopic structures fairly well, ultramicroscopically showed swelling and fragmentation of the cell membranes and a coagulative change of the matrix, and the ABO group activities were detectable in the cells above. Monoclonal antibodies could be produced against blood group antigen-related substances; anti-human red cell membrane and anti-human salivary blood group substance.
2. Forensic toxicological studies on severely charred bodies:
On volatile and gaseous poisons, fundamental experiments showed that ethanol and toluene remained in charred egg white masses, their contents being correlated with the thermochanges of the portions examined. In the experiments using animal carcasses, it was shown that ethanol, toluene, propane, ether and chloroform were detectable from charred organs. for the analysis of volatile poisons from traces of specimens, a new method was developed employing a curie-point pyrolyser.
Chlorpromazin, diazepam and methamphetamine proved to be determinable by GC-MS from nearly charred tissue specimens. Less

  • Research Products

    (44 results)

All Other

All Publications (44 results)

  • [Publications] OHSHIMA. T.: Proc. 13th Congr. Int. Acad. Forens. Soc. Med., Budapest, 1985.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] NAGANO,T.: Zbl. Rechtsmed.27. 854 (1985)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大島 徹: 日本法医学雑誌. 39. 100 (1985)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田中 宣幸: 日本法医学雑誌. 40. 726 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大島 徹: 日本法医学雑誌. 40. 647 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大島 徹: 日本法医学雑誌. 41. 171 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田中 宣幸: 日本法医学雑誌. 41. 173 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大島 徹: 日本法医学雑誌. 41. 713 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 高安 達典: 日本法医学雑誌. 41. 565 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 高安 達典: 日本法医学雑誌. 41. 661 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 木村 章彦: 日本法医学雑誌. 41. 555 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] TAKAYASUf,T.: Abstracts of 24th Int. Meet. Int. Ass. Forens. Toxicol.,Banff, 1987. 15 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] NAGANOf,T.: J.Can. Soc. Forens. Sci.20. 306-307 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] NAGANO,T.: Zbl. Rechtsmed.30. 465 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] OHSHIMA,T.: Acta Histochem. Cytochem.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 藤岡 良憲: 日本法医学雑誌. 42. (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 木村 章彦: 日本法医学雑誌. 42. (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] OHSHIMAf,T.: J. Leg. Med.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 永野 耐造: 日本法医学雑誌. 42. (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大島 徹: 日本法医学雑誌. 42. (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 笠井 謙多郎: 日本法医学雑誌. 42. (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松村 父征生: 日本法医学雑誌. 42. (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] OHSHIMA, T.: "Immunohistochemical study of blood group activities in human gastrointestinal tissues in normal and pathological conditions." proc. 13th Congr. Int. Acad. Forens. Soc. Med., Budapest, 1985.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] NAGANO, T.: "Immunhistochemische Untersuchung von Blutgruppenaktivitaten im menschlichen Magen und Darm." Zbl. Rechtsmed.27. 854- (1985)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] OHSHIMA, T.: "Studies on the distribution of blood group activities in human tissue - Blood group activities in gastrointestinal tract of normal and pathological state." Jpn. J. Leg. Med.39. 100- (1985)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] TANAKA, N.: "Activity changes of the antigens on the red cell membranes by heat." Jpn. J. Leg. Med.40. 726- (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] OHSHIMA, T.: "ABO(H)- and Lewis-activities in tissues and cells of Japanese monkey (macaca fuscata)." Jpn. J. Leg. Med.40. 647- (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] OHSHIMA, T.: "Immunocytochemical study on the ultrastructural localization of blood group activities in human tissue cells (I)." Jpn. J. Leg. Med.41. 171- (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] TANAKA, N.: "Activity changes of the antigens on the red cell membranes by heat (II)." Jpn. J. Leg. Med.41. 173- (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] OHSHIMA, T.: "Immunocytochemical study on the ultrastructural localization of blood group activities in human tissue cells (II)." Jpn. J. Leg. Med.41. 713- (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] TAKAYASU,T.: "Postmotrem changes and forensic toxicology. I. Analysis of ethanol and toluene from the experimentally decomposed carcasses." Jpn. J. Leg. Med.41. 565- (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] TAKAYASU, T.: "Postmortem changes and forensic toxicology. Experimental studies on II. Methamphetamine and III. Tranquilizers." Jpn. J. Leg. Med.41. 661- (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] KIMURA, A.: "On monoclonal antibodies produced by injection of red cell stroma into fool pad of mouse." Jpn. J. Leg. Med.41. 555- (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] TAKAYASU, T.: "Analysis of ethanol and toluene from the expermentally decomposed animal carcasses." Abstracts of 24th Int. Meet. Int. Ass. Forens. Toxicol., Banff, 1987. 15- (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] NAGANO, T.: "Analysis of some volatile poisons and drugs from the experimentally decomposed animal carcasses." J. Can. Soc. Forens. Sci.20. 306-307 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] NAGANO, T.: "Analytische Bestimmung einiger Gifte und Medikamente an den experimentell zur Faulnis gebrachten Tierkadavern." Zbl. Rechtsmed.30. 465- (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] OHSHIMA, T.: "Immunocytochemical localization of human-type ABO(H) blood group activity in a crab-eating macaque and bull frogs." Acta Histochem. Cytochem.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] FUJIOKA, Y.: "Extraction of some poisons and drugs from biological materials. I. Tranquilizer." Jpn. J. Leg. Med.42. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] KIMURA, A.: "On monoclonal antibodies for blood group substances in human saliva." Jpn. J. Leg. Med.42. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] OHSHIMA, T.: "Immunocytochemical study on the ultrastructural localization of human-type ABO(H)-blood group activities in a macaque (macaca irus)." J. Leg. Med.(1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] NAGANO, T.: "A new method for ethanol determination using a curie point pyrolyser." Jpn. J. Leg. Med.42. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] OHSHIMA, T.: "Immunohistochemical study on the localization of antigen defined by a human saliva-specific mouse monoclonal antibody." Jpn. J. Leg. Med.42. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] KASAI, K.: "Medico-legal analysis of volatile poisons from severely burned organs. Experimental study on ether." Jpn. J. Leg. Med.42. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] MATSUMURA, F.: "Identification and blood grouping of saliva from mixed body fluid by monoclonal antibodies against human saliva." Jpn. J. Leg. Med.42. (1988)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1989-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi