1987 Fiscal Year Final Research Report Summary
Determination of PGI_2 regulating factors and regulations of arachidonic acid in ischemic heart diseases
| Project/Area Number |
60440109
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Kyoto University |
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Principal Investigator |
KAWAI Chuichi Third Division, Department of Internal Medicine, Faculty of Medicine, Kyoto University (professor), 医学部・第3内科, 教授 (70025659)
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| Co-Investigator(Kenkyū-buntansha) |
TOSHIAKI Kumada same (assistant professor), 医学部・第3内科, 講師 (70127760)
HISYOSHI Fujiwara same (assistant professor), 医学部・第3内科, 講師 (80115930)
HIROFUMI Kambara same (associate professor), 医学部・第3内科, 助教授 (50109005)
YUI Yoshiki the same institute (Instructior), 医学部・第3内科, 助手 (20158330)
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| Project Period (FY) |
1985 – 1987
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| Keywords | AMI / HDL / LDL / Prostacyclin / 心筋梗塞 |
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| Research Abstract |
In this project, we have tried to determine the unknown theree regulators of prodtacyclin (PGI_2). There are three reg ulators; 1.PGI_2 synthesis stimulating factor, 2.PGI_2 stabilizing factor, 3. PGI_2 synthesis suppression foactor These factors are reported to be closely related to the pathogenesis of the thromboembolic disorders.
1. Stimulating factor;
The molecular weight of this factor is under 1,000. N@ mR study strongly suggest a possibility of sugar derivatives.
2. Stabilizing factor;
This factor has been purified to a single protein with a molecular weight of 28,000 dalton. Emplyed mwthods are Blue-Sepharose CL-6B chromatography, Sephacryl s-300 chromatography, and high-performance liquid chromatography by GS-620P. The N-terminal analysis revealed that this protein is identical to human apolipoprotein A-I, an apoprotein of HDL. PGI_2 specifically binds to HDL and does not bind to LDL and VLDL. This finding will contribute greatly to the clarification of the antiatherogenic action of HDL.
3. Suppression factor; This factor was purified as 60,000 D protein.
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