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1986 Fiscal Year Final Research Report Summary

Studies on hemopoietic factors produced by human neoplasms.

Research Project

Project/Area Number 60480155
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionSchool of Medicine, Tokai University

Principal Investigator

TAMAOKI Norikazu  School of Medicine, Tokai University, 医学部, 教授 (50055860)

Co-Investigator(Kenkyū-buntansha) HABU Sonoko  School of Medicine, Tokai University, 医学部, 助教授 (30051618)
HATA Jun-ichi  School of Medicine, Tokai University, 医学部, 教授 (90051614)
TADA Nobuhiko  School of Medicine, Tokai University, 医学部, 講師 (80167494)
UEYAMA Yoshito  School of Medicine, Tokai University, 医学部, 講師 (30072408)
Project Period (FY) 1985 – 1986
KeywordsHemopoietic factor / Colony stimulating factor / G-CSF / B cell growth factor <I> / Human tumor / ヒトがん / ヌードマウス
Research Abstract

For studying the pathogenesis of leukocytosis occasionally associated with neoplasia, 11 lines of human tumor transplantable to nude mice were established from cases with neoplasia and leukocytosis more than <10^4> / <mm^3> . These tumors included carcinoma originated from oral cavity, thyroid, lung and pancreas, leiomyosarcoma and myeloblastoma associated with CML and the extracts of these tumors showed CSF activity in vitro. A granulocyte colony stimulating factor (G-CSF) purified from the culture cell line of above oral cavity carcinoma showed prominent neutrophilia in vivo as well as G-CSF activity in vitro. The rabbit antibody to G-CSF which neutralized CSF activity in vitro demonstrated reaction products within endoplasmic reticulum and perinuclear space of original cell line. These findings indicates that G-CSF produced by human tumors directly stimulates host bone marrow cells and induces neutrophilic granulocytosis. In addition, nude mice bearing myeloblastoma and leiomyosarcoma showed prominent increase in splenic lymphocytes, B cells, plasma cells and serum IgM level. The supernatant of myeloblastoma in short term culture showed BCGF- <I> activity to mouse B cells triggered by anti- <mu> antibody.
Above results demonstrate that human neoplasms are able to produce various growth and differentiating factors to myeloid and lymphoid series and the human tumors transplantable to nude mice provide useful research resources for the study of hemopoietic factors produced by neoplasms.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] 秦順一: 病理と臨床. 3. 400-407 (1985)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nomura,H.: The EMBO Journal. 5. 871-876 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Satoh,N.: J.Cell Physiol.124. 255-260 (1985)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tamauchi,H.: Tokai J.Exp.Clin.Med.10. 621-629 (1985)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tada,N.: Immunogenetics. 24. 275-277 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 玉置憲一: 日病会誌. 73. 93-125 (1985)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 玉置憲一: "現代病理学大系9A「腫瘍概念,腫瘍病理,腫瘍細胞¨" 中山書店, 434 (1985)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 上山義人: "生化学実験講座 続8巻「血球分化¨" 東京化学同人, (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nomura, H.: "Purification and characterization of human granulocyte colony-stimulating factor (G-CSF)." EMBO Journal. 5. 871-876 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Satoh, N.: "Characterization of neutrophil alkaline phosphatase-inducing factor (AP-IF)." J. Cell Physiol.124. 255-260 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tamauchi, H.: "The in vivo role of murine natural killer cells in the development of B cell lineage in bone marrow." Tokai J. Exp. Clin. Med.10. 621-629 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tada, N.: "A new mouse lymphocyte alloantigen (Ly-28) defined by monoclonal antibodies." Immunogenetics. 24. 275-277 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tamaoki, N.: "Pathology of human tumors transplanted in nude mice - An experimental approach to the pathology of paraneoplasia (in Japanese)" Trans. Soc. Pathol. Jap.73. 93-125 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tamaoki, N.: Nakayama-Shoten, Tokyo. Host effects of neoplastic cells., 434 pages (1985)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1988-11-10  

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