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1987 Fiscal Year Final Research Report Summary

Age-related changes of the immune functions;Proliferative and differetiative activities of B and T lymphocytes

Research Project

Project/Area Number 60480202
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 内科学一般
Research InstitutionOsaka University

Principal Investigator

KISHIMOTO Susumu  Faculty of Medicine,Osaka University, 医学部, 教授 (60028420)

Co-Investigator(Kenkyū-buntansha) YOSHIZAKI Kazuyuki  The same as above, 医学部, 助手 (90144485)
HARA Hideki  The same as above, 医学部附属病院, 医員 (10211492)
NEGORO Shigeru  Faculty of Medicine,Osaka University, 医学部, 講師 (30172753)
Project Period (FY) 1985 – 1987
KeywordsAgeing / Aged / T cells / B cells / Proliferation / Differentiation / ルーマレセプター / B細胞分化因子
Research Abstract

Cytokinetic analyses for the proliferative response of T cells were done with use of colchicine to clarify the mechanisms of declined proliferation of T cells from the aged persons.The decreased ability to repeat replication was thought to responsible to the declined proliferation of aged T cells. The number of IL-2 binding sites was decreased on T cells from the aged. Decrement of the number of high affinity IL-2 binding sites was more pronounced than that of low affinity ones.The response of Tac-positive T cells to recombinant IL-2 was also decreased in T cells from the aged.We examined the proliferative capacity of CD4 positive T cells and CD8 positive T cells.CD8 positive T cells showed significantly lower response to combined stimulation of phorbol-12-myristate-13-acetate and ionomycin.
Increased production of B cell differentiation factor.whereas decreased production of IL-2,was first reported in our paper.
Proliferative capacity of B cells from the aged and the young were examined to find the declined proliferation of B cells from the aged.Cytokinetic analyses disclosed the decreased ability of B cells from the aged to repeat replication.Differentiative activity of B cells in response to given amount of B cell differentiation factor was determined to find increased activities among B cells drom the aged.It was not unreasonale to think that decreased proliferative capacity was compensated by increased production of B cell differentiation factor and increased activity of B cells to differentiate in response to the factor.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] S. Negoro: Mech. Agcing and Dev.36. 223-241 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H. Hara: Mech. Ageing and Dev.38. 245-258 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S. Negoro: Mech. Agcing and Dev.39. 263-279 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S. Negoro: Jpn. J. Geriat. 24. 93-99 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y. Deguchi: Mech. Ageing and Dev.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H. Hava: Submitted to Mech. Ageing and Dev.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Negoro: "Mechanism of age-related decline in antigen-specific T cell proliferative response:IL-2 receptor expression and recombinant IL-2 induced proliferative response of purified Tac positive T cells." Mech.Ageing Dev.36. 223-241 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Hara: "Age-associated changes in proliferative and differentiative response of human B cells and production of T cell-derived factors regulating B cell functions." Mech.Ageing Dev.38. 245-258 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Negoro: "Age-related changes of the function of T cell subsets:Predominant defect of the proliferative response in CD8 positive T cell subset in aged persons." Mech.Ageing Dev.39. 263-279 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Negoro: "Age-related functional and proportional changes in human lymphocytes: Clinical significance as indicators for immunosenescence." Jpn.J.Geriat.24. 93-99 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Deguchi: "Age-related changes of proliferative response,kinetics of expression of protooncogenes after the mitogenic stimulation and methylation level of the protooncogene in purified human lymphocyte subsets." Mech.Ageing Dev.in press.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Hara: "Age related changes of expression ofIL-2 receptor subunits and kinetics of IL-2 internalization in T cells after mitogenic stimulation." submitted.to Mech.Ageing Dev.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1989-03-30  

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