1986 Fiscal Year Final Research Report Summary
Preparation of human monoclonal antibodies using human B cell producing autoantibodies and analyses of novel autoantigens.
| Project/Area Number |
60480204
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
内科学一般
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| Research Institution | Sapporo Medical College |
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Principal Investigator |
YACHI Akira Sapporo Medical College, Department of Medicine, 医学部, 教授 (50045324)
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| Co-Investigator(Kenkyū-buntansha) |
HINODA Yuji Sapporo Medical College, Department of Medicine, 医学部, 助手 (10165128)
IMAI Kohzoh Sapporo Medical College, Department of Medicine, 医学部, 講師 (60117603)
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| Project Period (FY) |
1985 – 1986
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| Keywords | Autoantibody / Autoantigen / Human monoclonal antibody / エピトープ |
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| Research Abstract |
In some cases with autoimmune diseases and malignancies, autoantibodies to unknown antigens were detected in the sera. Using monoclonal antibodies, we have studied these autoantigens and autoantibodies and analysed the immune system. In order to prepare human type monoclonal antibodies, human B cells from cases with autoimmune diseases and malignancies were used. Therein we experienced a colonic cancer patient with anti-CEA monoclonal antibody. For parent cells, human myeloma cell line ARH77, human plasma cell line UC729-6 and mouse myeloma cell line P3-X63-Ag8-653 were served. Human cell lines which we used for fusion gave little hybridomas, but many human monoclonal antibodies were obtained when P3-X63-Ag8-653 was used. By a fusion with B cells in pleural effusion of a gastric cancer patient, human monoclonal antibody 3B7 (IgG) was raised, showing a staining reactivity with gastric and colonic cancers but not with non cancerous tissues. Molecular profile of autoantigen to which monoclonal antibody 3B7 reacts is under a further investigation. At present we are trying to raise hybridomas from new parent cells KR-4 and KR-12 using a newly developed electrofusion method.
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Research Products
(16 results)
