1987 Fiscal Year Final Research Report Summary
Research for the mechanism of tissue injury in tissue non-specific autoimmune diseases - the nature and the antigenecity of plasma DNA -
Project/Area Number |
60480205
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Research Category |
Grant-in-Aid for General Scientific Research (B)
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Allocation Type | Single-year Grants |
Research Field |
内科学一般
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Research Institution | Keio University |
Principal Investigator |
HOMMA Mitsuo Keio University, School of Medicine. Professor., 医学部・内科学, 教授 (20051047)
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Co-Investigator(Kenkyū-buntansha) |
KABURAKI Junichi Keio University, School of Medicine. Chief Physician., 医学部・内科学, 助手 (40175287)
TOMINAGA Norihiro Keio University, School of Medicine. Visiting Assistant Professor., 医学部・内科学, 客員講師
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Project Period (FY) |
1985 – 1987
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Keywords | Autoimmune diseases / SLE (Systemic lupus erythematosus) / Collagen diseases / DNA |
Research Abstract |
The nature, the origin and the concentration of plasma DNA were analysed in 37 patients with systemic lupus erythematosus (SLF) and 22 controls, including 16 patients with other diseases and 6 normals. DNA was extracted from 10.0ml of plasma by repeated phenol extraction after digestion with protease and RNase-A. The extracted DNA showed a single band with approximate base pairs of 10K. on the agarose gel electrophoresis. It was not digested by S1 nuclease, but was completely digested by DNase I, indicating double stranded DNA structure. The origin of plasma DNA was confirmed to be from human tissues by demonstrating the Alu family DNA sequence in the structure. The concentration of plasma DNA above 13ng/ml could be measured by the molecular hybridization technigue. Plasma DNA was positive in 8 out of 37 patients with SLE. All patients except one with positive plasma DNA were clinically active. In patients with other diseases, however, plasma DNA could be also demonstrated positive in 7, who had the suspect of tissue damages. Plasma DNA was negative in normals. Follow up study about patients with positive plasma DNA indicated that plasma DNA could be participated in the production of circulating immune complexes. Small fragments of 4% polyethylene glycol precipitated DNA could hybridize with plasma DNA. These data supported the concept that plasma DNA might play a significant role in the pathogenesis of SLE.
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Research Products
(14 results)