1986 Fiscal Year Final Research Report Summary
MICROCALORIMETRIC STUDY OF BRAIN TUMOR CELLS AND IT'S APPLICATION TO TUMOR DIAGNOSIS AND TREATMENT
| Project/Area Number |
60480330
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | MEDICAL COLLEGE OF OITA |
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Principal Investigator |
MORI TERUAKI MEDICAL COLLEGE OF OITA ASSOCIATE PROFESSOR, 医学部, 助教授 (60124632)
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| Co-Investigator(Kenkyū-buntansha) |
SUGIYAMA SATORU MEDICAL COLLEGE OF OITA . LECTURER, 医学部, 講師 (50154498)
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| Project Period (FY) |
1985 – 1986
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| Keywords | Micro-Calorimeter / Brain tumor cells / Cell cycle Anticancer drug / 発熱量 |
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| Research Abstract |
Living cell produces always bioenergy. but it is very difficult to meas ure bioenergy as cell lives in vitro.this time, we could measure bioene rgy of brain tumor cells(BTC) indirectory as microcaloric reaction in vitro by Micro-Calorimeter(ESCO,BC-01. DENSIKAGAKU CO.LTD.) In this study, it was investigated that microcalorimetry of many strains of BTC, microcaloric reaction of rat glioma cells(RG) to anticancer drug and irradiation, and its mechanism.
Consequently,it was proved that heat production ( HP )of 20 strains of BTC is different each other. HP of meningioma cells(n=4) was the largest (46.8+19.3 uw / 3x10 cells) as well as its diameter. HP of BTC increas ed straightaway acording as cell number increased. Both Procain and <NaN_3> decreased HP of RG. ADP and Con A increased it within one hour. ACNU,ADRIAMYCIN and IFN increased gradually HP of BTC about 30 - 50 % and the peak of HP was after 24 hours, then RG were accumulated in S and <G_2> - M phase as a result of analysis by flow cytometry (FACS 440). Irradiation effect to HP of monolayer RG showed the same tendency to ADM Microcalorimetry of our method will become a clue in analysis of specific character of BTC and be able to apply to clinical study.
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