1987 Fiscal Year Final Research Report Summary
Experimental and clinical study on entrapment neuropathy.
| Project/Area Number |
60480342
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Keio University |
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Principal Investigator |
UCHINISHI Kein-ichiro Keio Univ. School of Medicine Depart of Orthopaedic Surgery.Assist Prof., 医学部・整形外科学教室, 講師 (30051395)
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| Co-Investigator(Kenkyū-buntansha) |
NEMOTO Tetsuo Keio Univ. School of Medicine Depart of Orthopaedic Surgery.Instructor, 医学部・整形外科学教室, 助手 (80156166)
TAKAYAMA Shin-ichiro Keio Univ. School of Medicine Depart of Orthopaedic Surgery.Instructor, 医学部・整形外科学教室, 助手 (40138045)
HORIUCHI Yukio Keio Univ. School of Medicine Depart of Orthopaedic Surgery.Instructor, 医学部・整形外科学教室, 助手 (10138125)
ITOH Yoshiyasu Keio Univ. School of Medicine Depart of Orthopaedic Surgery.Assist Prof., 医学部・整形外科学教室, 講師 (90101948)
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| Project Period (FY) |
1985 – 1987
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| Keywords | entrapment neuropathy / compression neuropathy / compression apparatus neurofilament / tublin / 神経血管関門 / 神経周膜 / 神経圧迫装置 |
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| Research Abstract |
Compression is one of the most important causes of entrapment neuropathy. To elucidate the pathogenesis of compression neuropathy, we performed experimental study with the sciatic nerve of mongrel dogs. An apparatus devised to compress the nerve constantly with the force of 27.6mmHg was applied to the sciatic nerve.
The electroconductability was maintained up to the 8th weeks, but the motor nerve conduction velosity was reduced to approximately 60% of the control. Histologically, Wallerian degeneration was localized in the large myelinated fibers at the distal site of compression. In the teased single fiber specimens, paranodal demyelination was seen from the lst week and up to the 8th weeks. Such demyelination was more remarkable and a bulbous deformity appeared at the proximal site. To observe the deterioration of the axonal transport after compression neurofilament and tublin were seleced as a marker protein. These protein were stained specifically by monoclonal antibody with avidin biotin peroxidase complex method. Neurofilament was decrease in lst week of the distal site and gradually decreased. But this protein did not disappear even in 8th weeks. Tublin was decrease in lst week similar neurofilament matter.
The vulnerability of the peripheral nerve was mentioned in the pregnancy. To study the relationship, we produced subacute compression neruopathy of the sciatic nerve of pregnant dogs. The electroconductability of pregnant dog and control were nearly equal. In pregnant group, complete conduction block was induced in 50% and mean M.C.V.was 26.2% at 3weeks. In the hormonal investigation, serum concentration of estrogen was slightly higer and progesterone was lower in pregnant. On the equal compression force, severe paralysis was found in pregnant. This penomenon proved that the peripheral nerve was vulnerable associated with pregnancy.
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