1986 Fiscal Year Final Research Report Summary
The immune response to periodontopathic bacteria in experimental periodontitis of dogs
| Project/Area Number |
60480410
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Conservative dentistry
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| Research Institution | Niigata University |
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Principal Investigator |
HARA Kohji Niigata University School of Dentistry Professor, 歯学部, 教授 (20018419)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAZAKI Kazuhisa Niigata University Dental Hospital Clinical Instructor, 歯学部附属病院, 助手 (00182478)
YOSHIE Hiromasa Niigata University School of Dentistry Associated Professor, 歯学部, 助教授 (20143787)
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| Project Period (FY) |
1985 – 1986
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| Keywords | Actinobacillus actinomycetemcomitans ( Aa ) <Y_4> / Anti Aa antibody / Experimental periodontitis / Injection of antibody / Topical application of antibody / Complement mediated bactricidal activity / イヌ好中球 |
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| Research Abstract |
The purpose of this study was to investigate the susceptibility of Actinobacillus actinomycetemcomitans ( Aa ) <Y_4> to anti Aa antibody.
First, in vivo assay was carried. Experimental periodontitis in dog was established by removal of the alveolar bone and wire ligature around teeth. Each 0.3 ml of antibody, sham antibody or PBS was injected on buccal attached gingiva, or 5 <micro> l of each was dropped in periodontal pocket 4 times. Nothing was carried out in control sites, and 2 <micro> l Aa suspension, adjusted to the concentration of 1 x <10^(11)> Aa/ml, was dropped on all sites. After 4 hours, contents of each pocket was collected by crevicular washing and cultured anaerobically. Total colony forming unit ( CFU ) of Aa was calculated and PMN leukocytes was counted.
Next, we have examined how the antibody influenced on complement mediated bactericidal activity and phagocytic activity of dog PMN leukocyte in vitro.
The results were as follows;
The bactericidal activity and the activity of accelerating PMN leukocyte migration of the antibody was not recognized. But the significant reduction of Aa CFU was recognized when that was incubated with antibody, complement and PMN leukocytes, and not stirred up. When antibody was included in the incubation, PMN leukocytes tended to phagocytize Aa cells compared to antibody free culture. This suggests that the antibody, comlement and PMN leukocyte system was capable of aggregating or phagocytizing Aa, but not killing.
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