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1987 Fiscal Year Final Research Report Summary

Studies on Intra-cellular New Electron Transfer Systems Containing Aldehyde Oxidase as a Component.

Research Project

Project/Area Number 60480462
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Biological pharmacy
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

TATSUMI Kiyoshi  Professor, Hiroshima University School of Medicine, 医学部, 教授 (90037581)

Co-Investigator(Kenkyū-buntansha) KITAMURA/SHIGEYUKI  Research Assistant, Hiroshima University School of Medicine, 医学部, 助手 (40136057)
YOSHIHARA/SHIN'ICHI  Associate Professor, Hiroshima University School of Medicine, 医学部, 助教授 (00037607)
Project Period (FY) 1985 – 1987
Keywordsnew electron transfer system / aldehyde oxidase / flavin enzyme / drug metabolism / drug-reducing activity / rabbit / guinea pig
Research Abstract

Neither xanthine oxidase, xanthine dehydrogenase, lipoamide dehydrogenase, NADPH-cytochrome c reductase nor NADH-cytochrome b reductase by itself exhibited reductase activities towards N-oxide and sulfoxide ^5 compounds even in the presence of its electron donor. However, when one of these flavin enzymes and its electron donor were combined with aldehyde oxidase, significant N-oxide and sulfoxide reductase activities were observed in either case. This fact indicated that aldehyde oxidase is capable of coupling with such flavin enzymes as described above, forming new electron transfer systems in which N-oxide and sulfoxide compunds function as final electron acceptors.
Rabbit live aldehyde oxidase has been found to utilize as electron acceptors aromatic hydroxamic acids, N-aryl aliphatic hydroxamic acids and oximes, as well as N-oxide and sulfoxide compounds. In the present study, furthermore, aldehyde oxidase was purified successfully from guinea pig liver and bovine ciliary body. In either case, the purified enzyme was homogeneous by the criterion of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS- PAGE). Its molecular weight was estimated to be about 300,000 by gel filtration and to be about 150,000 by SDS-PAGE.

  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Shigeyuki Kitamura: Biochimical and Biophysical Research Communicationc. 133. 67-74 (1985)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shin'ichi Yoshihara: Archive of Biochemistry and Biophysics. 242. 213-224 (1985)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shin'ichi Yoshihara: Journal of Pharmacobio-Dynamics. 8. 996-1005 (1985)

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      「研究成果報告書概要(和文)」より
  • [Publications] Kiyoshi Tatsumi: Cancer Research. 46. 1089-1093 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shin'ichi Yoshihara: Archives of Biochimestry and Biophysics. 249. 8-14 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kiyoshi Tatsumi: Biochemical and Biophysical Research Communication. 138. 312-317 (1986)

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      「研究成果報告書概要(和文)」より
  • [Publications] Kazumi Sugihara: Archives of Biochemistry and Biophysics. 247. 289-293 (1986)

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      「研究成果報告書概要(和文)」より
  • [Publications] Kiyoshi Tatsumi: Eisei Kagaku. 32. P-3 (1986)

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      「研究成果報告書概要(和文)」より
  • [Publications] Kiyoshi Tatsumi: Journal of Pharmacobio-Dynamics. 9. S-26 (1986)

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      「研究成果報告書概要(和文)」より
  • [Publications] Kiyoshi Tatsumi: Archives of Biochemistry and Biophysics. 253. 413-418 (1987)

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      「研究成果報告書概要(和文)」より
  • [Publications] Shigeaki Shimada: Investigative Ophthalmology & Visual Sciense. 28. 1204-1206 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kiyoshi Tatsumi: Biochemecal and Biophysical Research Communication. 142. 376-382 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kiyoshi Tatsumi: Eisei Kagaku. 33. P-8 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kiyoshi Tatsumi: Journal of Pharmacobio-Dynamics. 10. S-82 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shigeyuki Kitamura and Kiyoshi Tatsumi: "Purification of N-Hydroxy-2-acetylaminofluorene Reductase from Rabbit Liver Cytosol" Biochemical and Biophysical Research Communication. 133. 67-74 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shin'ichi Yoshihara and Kiyoshi Tatsumi: "Guinea Pig Liver Aldehyde Oxidase as a Sulfoxide Reductase. Its Purification and Characterization" Archives of Biochemistry and Biophysics. 242. 213-224 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shin'ichi Yoshihara and Kiyoshi Tatsumi: "Sulfoxide Reduction Catalyzed by Guinea Pig Liver Aldehyde Oxidase Coupled with One-electron Reducing Flavoenzymes" Journal of Pharmacobio-Dynamics. 8. 996-1005 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kiyoshi Tatsumi, Shigeyuki Kitamura and Noriko Narai: "Reductive Metabolism of Aromatic Nitro Compounds Including Carcinogens by Rabbit Liver Preparations" Cancer Research. 46. 1089-1093 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shin'ichi Yoshihara and Kiyoshi Tatsumi: "Kinetic and Inhibition Studies on Reduction of Diphenylsulfoxide by Guinea Pig Liver Aldehyde Oxidase" Archives of Biochemistry and Biophysics. 249. 8-14 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kiyoshi Tatsumi and Shigeyuki Kitamura: "N-Hydroxy-2-acetylaminofluorene as Effective Inhibitor of Aldehyde Oxidase" Biochemical and Biophysical Research Communication. 138. 312-317 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kazumi Sugihara and Kiyoshi Tatsumi: "Participation of Live Aldehyde Oxidase in Reductive Metabolism of Hydroxamic Acids to Amides" Archives of Biochemistry and Biophysics. 247. 289-293 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kiyoshi Tatsumi, Shigeyuki Kitamura, Noriko Narai and Hirotaka Amano: "Studies on Reductive Metabolism of Aromatic Nitro Compounds" Eisei Kagaku. 32. P-3- (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kiyoshi Tatsumi and Shigeyuki Kitamura: "Enzymes Involved in Dehydroxylation of a Carcinogen N-Hydroxy-2-acetylaminofluorene" Journal of Pharmacobio-Dynamics. 9. S-26- (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kiyoshi Tatsumi and Masaki Ishigai: "Oxime-Metabolizing Activity of Liver Aldehyde Oxidase" Archives of Biochemistry and Biophysics. 253. 413-418 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shigeaki Shimada, Hiromu Mishima, Shigeyuki Kitamura and Kiyoshi Tatsumi: "Nicotinamide N-oxide Reductase Activity in Bovine and Rabbit Eyes" Investigative Ophthalmology & Visual Science. 28. 1204-1206 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kiyoshi Tatsumi and Hirotaka Amano: "Biotransformation of 1-Nitropyrene and 2-Nitrofluorene to Novel Metabolites, the Corresponding Formylamino Compounds, in Animal Bodies" Biochemical and Biophysical Research Communication. 142. 376-382 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kiyoshi Tatsumi, Hirotaka Amano, Kenji Ueda and Chieko Maegawa: "Metabolism of Nitropolycyclic Aromatic Hydrocarbons and Related Compounds" Eisei Kagaku. 33. P-8- (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kiyoshi Tatsumi and Masaki Ishigai: "Mechanism for Biotransformation of an Aliphatic Amine via the Corresponding Oxime" Journal of Pharmacobio-Dynamics. 10. S-82- (1987)

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      「研究成果報告書概要(欧文)」より

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Published: 1989-03-30  

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