1986 Fiscal Year Final Research Report Summary
Studies on Protease Inhibitor Isolated from Breast Cancer and Breast Cancer Cell Line
| Project/Area Number |
60480518
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
NISHIKI Masayuki Hiroshima University School of Medicine, 医学部, 助教授 (70034014)
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| Co-Investigator(Kenkyū-buntansha) |
OKUMICHI Tsuneo Hiroshima University School of Medicine, 医学部附属病院, 助手 (10177181)
TAKEICHI Nobuo Hiroshima University School of Medicine, 医学部, 助手 (20034655)
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| Project Period (FY) |
1985 – 1986
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| Keywords | Breast caucer / Normal mammary gland / Breast cancer cell line / Purification / 精製 |
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| Research Abstract |
1) TPIs in human breast cancer extracts were significantly higher than in normal mammary gland extracts.
2) TPI was purified from both human breast cancer and human normal mammary gland extracts by papain-Sepharose affinity chromatography and Sephacryl S-200 gel filtration.Two kinds of TPIs (Iow-molecular weight TPI and high-molecular weight TPI) were purified from both tissues. Their molecular weights were 14,000 and 90,000,respectively as determined by gel filtration.
3) Low-molecular weight (LMW-) TPI had higher specific activity than high-molecular weight (HMW-) TPI. In breast cancer tissue extracts,LMW-TPI was dominant,Contrarily,HMW-TPI was dominant in normal gland tissue extracts.
4) Only HMW-TPI reacted with anti-urinary thiol protease inhibitor (UTPI) rabbit IgG by double immunodiffusion and immunoelectrophoresis.
5) LMW-TPI inhibited papain competitively using S-2302 as substrate.Its km and ki were 1.3x <10^(-3)> M and 6.1x <10^(-8)> M, respectively.
6) LMW-TPI was found to be stable to heat and pH variation.
7) TPIs were also purified from the human breast cancer cell line (YMB-l) . Both TPIs which were extracted from cultured cells and released into the medium, were confirmed to be LMW-TPI.
Breast cancer cells may have lower ability to produce HMW-TPI than normal mammary gland cells. The difference of antigenicity to anti-UTPI IgG between HMW-TPI andLMW-TPI may be useful for diagnosis in near future.
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Research Products
(8 results)
