1987 Fiscal Year Final Research Report Summary
Amino sugars and their related compounds having nucleic acid- scissoring activity.
| Project/Area Number |
60490024
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
広領域
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| Research Institution | Fukuoka University |
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Principal Investigator |
WATANABE kenji Fukuoka Univ., Foc.Pharm.Sci., Professor, 薬学部, 教授 (40078689)
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| Co-Investigator(Kenkyū-buntansha) |
KAKITA Yukari Fukuoka Univ., Fac,Pharm.Sci., Assistant, 薬学部, 副手 (80194679)
NAKASHIMA Yukihiko Fukuoka Univ., Foc.Pharm.Sci., Assistant., 薬学部, 助手 (50164174)
SUMOTO Kunihiro Fukuoka Univ., Fac.Pharm.Sci., Assistant Professor, 薬学部, 助教授 (00078677)
YAMAGUCHI Tadatoshi Miyazaki Med.Col., Dept.Hyg., Instructor, 講師 (80037598)
MIHASHI Kunihide Fukuoka Univ., Fac.Pharm.Sci., Assistant Professor, 薬学部, 助教授 (60078681)
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| Project Period (FY) |
1985 – 1987
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| Keywords | Aminosugars / D-glucosamine / DNA damage / DNA切断 / 活性酸素 / マキサム・ギルバート法 |
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| Research Abstract |
DNA strand scisson, change of ccc-DNA into oc- and linear-DNA and urther fragmented DNA, by various aminosugars was investigated through agarose gel electrophoresis.
D-Glucosamine, D-mannosamine, D-galactosamine and D-isoglucosamine had the DNA strand-scissoring activity. Both the C-2 amino group and C-1 aldehyde one of the molecules were indespensable for their activities. D-Glucosamine, auto- claved at 120゜C in agueous solution, had a greater activity than D-glucosamine itself. The reaction was stimulated by Cu^<2+> and inhibited by catalase, SOD and some radical scavengers. Some free radicals, particularly hydroxyl radical, generated during autoxidation of aminosugrs in agueous solution, were thought to be involved in the DNA strand strand scission. D-Glucosamine induced alkaline- be involved in the DNA strand strand scission. D-Glucosamine induced alkaline- lable damage preferentially at pyrimidine residues in the dinucleotide sequence of pyrimidine-purine (5'-> 3').
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