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1986 Fiscal Year Final Research Report Summary

Studies on prenylquinones in green leaves

Research Project

Project/Area Number 60540436
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 植物生理学
Research InstitutionKyushu University

Principal Investigator

OKAYAMA Shigeki  Kyushu University, College of Generall Education, 教養部, 教授 (50038444)

Project Period (FY) 1985 – 1986
Keywordsprenylquinone / plastoquinone / phylloquinone / tocopherolquinone / chloroplasts / HPLC / voltanmetry / ホウレン草
Research Abstract

Distributions and redox levels of prenylquinones in leaves and chloroplasts were studied using the methods developed here. The method is consisted of rapid extraction of the quinones on the sample preparation cartridge, followed by the separation and detection with the reversed-phase high-performance liquid chromatography using the dual-electrodes voltanmetric detector.
1. More than half of plastoquinones in thylakoid membranes were found in lipid matrix, suggesting its role as mobile electron and proton carrier. The rest of plastoquinones were in particles as fixed electron/proton carriers. The molar ratios of each derivatives to chlorophyll were almost the same in every part of thylakoid membrane. Phylloquinone was concentrated in photosytem I particle, possibly as a redox component. The ratio of tocopherolquinone to chlorophyll was almost constant in every portion of thylakoid membrane, suggesting its role as a protectant for the photoinjury of pigment systems by strong light.
2. During the development of spinach leaves the ratio of plastoquinone-C to chlorophyll was increased, and of ubiquinone-9 was decreased. The ratios of the other prenylquinones were not changed. It seems that the role of the respiratory system as ATP producing system is partly replaced by the photosynthetic system in the course of leaf development. Plasstoquinone C might be produced by photochemical epoxydation of plastoquinone A.
3. The primary photochemical reaction in Photosystem II required two plastoquinone A molecules.
4. The redox potential of plastoquinone A in chloroplasts was +20 mV at pH 7.8.

  • Research Products

    (5 results)

All Other

All Publications (5 results)

  • [Publications] 岡山繁樹: 九州大学中央分析センター報告. 4. 51-55 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Tabata;S,Itoh;Y.Yamamot;S.Okayama;M.Nishimura: Plant and Cell Physiology. 26. 855-863 (1985)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shigeki Okayama: "High-performance liquid chromatography of prenylquinones in green leaves using an electrochemical detector" Report of the center of advanced instrumental analysis, Kyushu University. 4. 51-55 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Tabata,S.Itoh,Y.Yamamoto,S.Okayama and M.Nishimura: "Two plastoquinone A molecules are required for photosystem II Activity: Analysis in hexane-extracted Photosystem II particles" Plant and Cell Physiology. 26. 858-863 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Isogai, M.Nishimura and S.Okayama: "Simultaneous determination of plastoquinone A and plastoquinol A by high-performance liquid chromatography with an electrochemical detector; A rapid and simple procedure for determination of the redox level of" plastoquinone A in spinach chloroplasts.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1988-11-10  

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