1987 Fiscal Year Final Research Report Summary
Physiological and biochemical studies on the cold-adaptation of the silkworm eggs.
| Project/Area Number |
60560064
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
蚕糸学
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| Research Institution | Kyoto Institute of Technology |
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Principal Investigator |
FURUSAWA Toshiharu associate Prof. Faculty of Textile Science, Kyoto Institute of Technology, 繊維学部, 助教授 (70127166)
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| Project Period (FY) |
1985 – 1987
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| Keywords | silkworm eggs / sorbitol / glycerol / lipid / alcohol / phosphoglyceride / permeability / 不飽和脂肪酸 |
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| Research Abstract |
The biochemical responses of Bombyx eggs to cold environment was examined from the viewoint of polyol and lipid metabolisms. The sorbitol accumulation was observed even in non-diapause eggs when these eggs were kept below 10゜C. However, the fluctuation of sorbitol would be different between temperatures of 1゜C and 5゜C. This phenomenon may be not due to phosphofructokinase activity but the difference of NAD-sorbitol dehydrogenase activity in the non-diapause eggs under lower temperatures.
As for lipids which would be associated with oxygen permeability of egg-shells on the initiation of diapause, GC-MS analysis indicated that they were consisted of two components: acetate esterified alcohols of chain length C_<32> and C_<52>, saturated. The predominant phosphoglyceride in silkworm eggs is phsphatidylcholone (PC), followed by Phosphatidylethanolomine (PE) and phosphatidyl serine (PS). PC, PE and PS contained palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1), linoleic acid (18:2) and linolenic acid (C18:3) as fatty acylchains. Unsaturated fatty acid in phosphoglyceride was changed in quality by chilling of the eggs under lower temperatures.
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