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1987 Fiscal Year Final Research Report Summary

Biochemical and molecular biological study on Subunit interaction of RuBisCO

Research Project

Project/Area Number 60560089
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 応用生物化学・栄養化学
Research InstitutionSchool of Agriculture, Nagoya University

Principal Investigator

TAKABE Tetsuko  School of Agriculture, Nagoya University, 農学部, 講師 (60089852)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Hirokazu  RI center, Nagoya University, アイソトープセンター, 助手 (80170348)
AKAZAWA Takashi  School of Agriculture, Nagoya University, 農学部, 教授 (20023400)
Project Period (FY) 1985 – 1987
KeywordsRuBP carboxylase / oxygenase / subunit interaction / gene expression and cloning / 塩ストレス
Research Abstract

(1) A DNA sequence containg genes for large and small subunits of ribulose 1, 5-bisphosphate carboxylase/oxygenase (RuBisCO) from photosynthetic purple sulfur bacterium Chromatium vinosum was cloned and sequenced. The cloned rb cLl and rbc S1 genes encode subunits of active plant-type RuBisCO which are barely expressed in Chromatium.
(2) Growth of halotolerant cyanobacterium Aphanothece halopytice, previously adapted to 0.5 M NaCl, was optimal when NaCl concentration was in the range0.5-1.0 M. The growth was delayed at either too low or too high salinities with lags of ca. 0.5 day in 0.25 M NaCl and ca. 2days in 2 M NaCl under the experimental conditions. However, the growth rates at logarithmic phase were similar in the culture media containing NaCl in the range 0.25-2.0 M. The capacity of photosynthetic CO_2 fixation increased in the cells at logarithmic phase as NaCl concentration in the culture medium increased. The protein level of ribulose 1,5-bisphosphate carboxylase/oxygenase was also found to increase under high salinities using both immunoblotting method and protein A-gold immunoelectron microscopy. These results suggest that high photosynthetic capacity and high RuBisCO content may play an important role in adaptation and cellular metabolisms of Aphanothece cells grown in high salinities.

  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] A.Incharoensakdi;T.Takabe and T.Akazawa: Arch.Biochem.Biophys.237. 445-453 (1985)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Incharoensakdi;T.Takabe;Teruhiro Takabe and T.Akazawa: Biochem.Biophys,Res,Commun.126. 698-704 (1985)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Incharoensakdi;T.Takabe and T.Akazawa: Arch.Biochem.Biophys.248. 62-70 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Incharoensakdi;T.Takabe;Teruhiro Takabe and T.Akazawa: Biochem.Biophys,Res,Commun.138. 118-124 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Incharoensakdi;T.Takabe and T.Akazawa: Plant Playsiol. 81. 1044-1049 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.M.Viale;H.Kobayashi;T.Takabe and T.Akazawa: FEBS Letters. 192. 283-288 (1985)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Kobayashi;A.M.Viale;T.Takabe: J.Biol.Chem.(1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Akazawa;A.Incharoensakdi;T.Takabe: Carbon dioxide as a Source of Carbon. 83-91 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 高倍鉄子: 日本農芸化学会誌. 59. 921-928 (1985)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 高倍鉄子,アラン インチヤロンサ: 生化学. 58. 175-182 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Incharoensakdi;T.Takabe: J.Sci.Soc.Thailand. 13. 133-137 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A. Incharoensakdi, T. Takabe and T. Akazawa: "Factors affecting the dissociation and reconstitution of ribulose-1,5-bisphosphate carboxylase/oxygenase from Aphanothece halophytica" Arch. Biochem. Biophys,. 237. 445-453 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A Incharoensakdi, T. Takabe, Teruhiro Takabe and T. Akazawa: "Heterologous hybridization of ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) restores the enzyme activities" Biochm. Biophys, Res, Commun.126. 698-704 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A. Incharoensakdi, T. Takabe and T. Akazawa: "Role of the small subunit of ribulose 1,5-bisphosphate carboxylase/oxygenase in the activation process" Arch. Biochem. Biophys.248. 62-70 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A. Incharoensakdi, T. Takabe, Teruhiro Takabe, and T. Akazawa: "Isolation of the catalytically competent small subunit of ribulose bisphosphate carboxylase/oxygenase from spinach under an extremely alkaline condition" Biochem. Biophys. Res. Commun.138. 118-124 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A. Incharoensakdi, T. Takabe and T. Akazawa: "Effect of betaine on enzyme activity and subunit interaction of ribulose 1,5-bisphosphate carboxylase/oxygenase from Aphanothece halophytica" Plant Playsiol,. 81. 1044-1049 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A. M. Viale, H. Kobayashi, T. Takabe and T. Akazawa: "Expression of genes for subunits of plant-type RuBis CO from Chromatium and production of the enzymically active molecule in Escherichia coli" FEBS Letters. 192. 283-288 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Kobayashi, A.M. Viale, T. Takabe, H. Fukada, E. Valle, K. Wada,K. Shinozaki, M. Sugiura and T. Akazawa: "DNA sequence encoding fully active plant-type ribulose 1,5-bisphosphate carboxylase/oxygenase from photosynthetic bacterium Chromatium vinosum" J. Biol. Chem.(1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Akawawa, A. Incharoensakdi, T. Takabe: "Ribulose 1,5-bisphosphate carboxylase/oxygenase(RuBisCO)(Isolation, structure and function)" Carbon dioxide as a Source of Carbon. 1987. 83-91

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A. Incharoensakdi and T. Takabe: "Structure, Function and regulation of ribulose 1,5-bisphosphate carboxylase/oxygenase" J. Sci. Soc. Thailand. 13. 133-137 (1987)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1989-03-30  

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