1986 Fiscal Year Final Research Report Summary

Regulation of glucose metabolism in chicken kidney

Research Project

Project/Area Number	60560323
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	基礎獣医学
Research Institution	University of Osaka Prefecture
Principal Investigator	SUGANO Tsukasa College of Agriculture, University of Osaka Prefecture, 農学部, 助教授 (30081516)
Project Period (FY)	1985 – 1986
Keywords	Chicken / Kidney / Liver / Perfusion / Gluconeogenesis / 糖分解 / 代謝
Research Abstract	The regulation of glucose metabolism was studied in chicken kidney perfused with either an arterial system or a portal system. A. Provided that kidneys were perfused with oxygenated buffer solution at a flow rate of 5 ml/min per g of kidney in both perfusion system, oxygenation of the kidney was achieved. B. Pyruvate, an oxidized substrate, found to be a much better gluconeogenic precursor than lactate in the perfused chicken kidney. C. Quinolinate, an inhibitor of cytosolic phosphoenolpyruvate carboxykinase, inhibited gluconeogenesis from lactate and pyruvate but not from succinate, malate and <alpha> -ketoglutarate. D. Chicken renal gluconeogenesis occurs in the cortex and glycolysis occurs in medullary structures. Furthermore, succinate, transported by a <Na^+> -dependent system and monocarboxylic acids transported by a <Na^+> -independent system through the basolateral side of the kidney are good substrates for gluconeogenesis.

(4 results)