1986 Fiscal Year Final Research Report Summary
Investigation on therapy of early stage of hepatic hydatid disease by using experimental animal model.
| Project/Area Number |
60570168
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
寄生虫学(含医用動物学）
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
KUTSUMI Haruhiko Asahikawa Medical College, 医学部, 教授 (90072868)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAO Minoru Asahikawa Medical College, 医学部, 助手 (70155670)
INAOKA Tohru Asahikawa Medical College, 医学部, 助手 (00091562)
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| Project Period (FY) |
1985 – 1986
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| Keywords | Echinococcosis / Experimental therapy / Mebendazole / Albendazole / Ivermectin / X-ray irradiation / Heat shock |
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| Research Abstract |
The effect of drugs, X-ray irradiation and heat shock on secondary multilocular echinococcosis in experimentally infected rodents was investigated. Among drugs used, mebendazole was most effective particularly when it was administered with intraperitoneal injection. Though mebendazole remarkably inhibited the growth of the hydatid it could not completely kill the parasite tissues. Albendazole was less effective than mebendazole. Many other drugs, ivermectin, praziquantel, cyclophosphamide, fluolouracil, mitomycin C, carmofur, norfloxancin, OK-432 and milbemycin D were ineffective. X-ray irradiation influenced on the proliferative ability of germinal layer cells of hydatid at dose level of 45,000 R or more. Germinal layer cells of hydatid lost their proliferative ability by the heat shock at 46C or above for 120 min.
The production of specific antibody (IgG) to larval Echinococcus multilocularis in experimentally infected rodents. It elevated from 2 weeks after infection in untreated animals while from 4 weeks after infection in the animals treated with mebendazole.
Larval E. granulosus was successfully isolated from sterile hydatid of Australian cattle origin. It is still maintained in our laboratory with intraperitoneal passages by using Chinese hamsters. This is the first successful case from sterile hydatid in E. granulosus. This isolate will be able to be used for experimental therapy in future studies as well as that of E. multilocularis.
More than 100 human cases of multilocular echinococcosis which have hitherto been reported from Japan were reviewed. The present status of the human multilocular echinococcosis was discussed.
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Research Products
(8 results)
