1986 Fiscal Year Final Research Report Summary
New Tn5 variants with various drug resistance markers and suicide vector for transposon mutagenesis
| Project/Area Number |
60570188
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
細菌学
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| Research Institution | University of Tokyo |
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Principal Investigator |
SASAKAWA Chihiro Institute of Medical Science, University of Tokyo, Associate Professor, 医科学研究所, 助手 (70114494)
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| Project Period (FY) |
1985 – 1986
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| Keywords | transposon / plasmid / 薬剤耐性 |
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| Research Abstract |
A series of variants of transposon Tn5 were constructed by replacement of the 2.7 Kb central segment which encodes kanamycin resistance with various other resistance genes : tetracycline( <Tc^r> ), chloramphenicol( <Cm^r> ), gentamicin( <Gm^r> ), trimethoprim( <Tp^r> ), streptomycin( <Sm^r> ) or ampicillin( <Ap^r> ). This set of new derivatives of Tn5 is useful for insertion mutagenesis and for the tagging of cryptic plasmids in diverse gram-negative bacteria. A thermosensitive replication mutant of the broad-host range transmissible plasmid R388 was also constructed for use as a suicide vector for the delivery of transposable elements.
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