1986 Fiscal Year Final Research Report Summary
Study on urinary mutagen in smokers
| Project/Area Number |
60570242
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Hygiene
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| Research Institution | Kyushu University |
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Principal Investigator |
ISHINISHI Noburu Kyushu University, Faculty of Medicine, Professor, 医学部, 教授 (80037340)
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| Co-Investigator(Kenkyū-buntansha) |
HIRATA Miyuki Kyushu University, Faculty of Medicine, Assistant, 医学部, 助手 (30156674)
YAMAMOTO Akiyo Kyushu University, Faculty of Medicine, Assistant, 医学部, 助手 (10136484)
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| Project Period (FY) |
1985 – 1986
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| Keywords | Mutagenicity of urine / Ames test / Smoker / シリカゲルカラムクロマトグラフィー |
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| Research Abstract |
The mutagenicity of urine from a healthy population of young adult men, including 61 smokers and 107 nonsmokers, was investigated with the Salmonella/microsome test using the tester strain S. typhymurium TA98. In quantifying the assay, the use of dichrolomethane was suitable for eluting the mutagenicity of smokers' urine from XAD-2 resin column. We assayed mutagenicity in condensates of such dichrolomethane eluates and found that the mutagenicity of smokers' urine was significantly higher than that of nonsmokers' urine. In the smokers' group, correlation coefficient between "the mutgenicity of the urine" and "the acutual number of cigarettes smoked on the day of urine collection" or "the average number of cigaretts smoked per day" was statistically significant.
Such mutagenic activity in smokers' urine was eluted in methanol fraction, when the urine condensates was chromatographed on silica gel(Wako gel S-1).
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