1986 Fiscal Year Final Research Report Summary
Biochemical and ultrastructural studies on glycosaminoglycans and glycoproteins of the lung in health and diseases
| Project/Area Number |
60570343
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
MOTOMIYA Masakichi Department of Medicine, The Research Institute for Tuberculosis and Cancer, 抗酸菌病研究所, 助教授 (20006092)
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| Co-Investigator(Kenkyū-buntansha) |
ARAI Hideo Department of Medicine, The Research Institute for Tuberculosis and Cancer, 抗酸菌病研究所内科, 助手 (90159484)
TAKUSAGAWA Kimihiko Department of Medicine, The Research Institute for Tuberculosis and Cancer, 抗酸菌病研究所内科, 助手 (10125601)
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| Project Period (FY) |
1985 – 1986
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| Keywords | glycosaminoglycan / chondroitin sulfate / fibronectin / lung cancer / mesothelioma / 肺癌 / サルコイドーシス / 胸膜炎 |
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| Research Abstract |
1) In experimental pleural effusion produced in guinea pigs, evidence which was suggestive of the synthesis of hyaluronic acid in the pleural tissue was obtained.
2) In view of the rarity of the occurrence of metastases to the brain from a primary mesothelioma, attempts were made to establish a method by which the diagnosis of primary mesothelioma plus brain metastasis could be confirmed. As a result, application of hyaluronidase from streptomyces hyalurolyticus proved to be useful for this purpose.
3) In the human lung, a possibility was suggested that chondroitin-6-sulfate was converted into chondroitins-4-sulfate and then into chondroitin.
4) Large cell carcinoma, small cell carcinoma and poorly-differentiated carcinoma of the human lung tended to be fibronectin(FN)-positive, as shown by an immunohistochemical study in which a polyclonal anti-FN-antibody was applied.
5) The fibrotic process of the paraquat lung was associated with an increase of glycosaminoglycans in the lung in terms of uronic acid.
6) Immunoelectron microscopic studies of FN of the human lung tissue revealed the presence of FN in endothelial, alveolar epithelial and bronchial epithelial basal laminae as well as in basal laminae of fibroblasts and smooth muscle cells.
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